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Separation of dithiocarbamate metal complexes by micellar electrokinetic chromatography

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posted on 2023-05-16, 20:04 authored by Emily HilderEmily Hilder, Miroslav MackaMiroslav Macka, Paul HaddadPaul Haddad
Separation of a number of transition metal complexes with bis(2-hydroxyethyl)dithiocarbamate (HEDTC) by micellar electrokinetic capillary chromatography (MEKC) with direct photometric detection was investigated. Pre-capillary complexation was shown to be superior to on-capillary complexation in terms of the number of metals ions which could be detected and their peak shapes, enabling analysis of Cd(II), Pb(II), Pt(II), Co(II), Ni(II), Bi(III), Cr(II), Cu(II) and Hg(II). The type and composition of the background electrolyte (BGE) were investigated with respect to separation selectivity and BGE stability. A BGE of 60 mM 3-(morpholino)propane sulfonic acid (MOPS), 30 mM tris(hydroxymethyl)aminomethane (Tris), 10 mM sodium dodecyl sulphate (SDS) and 100 μM HEDTC, pH 7.2 was found to be optimal with nine metals baseline separated in less than seven minutes, and giving separation efficiencies of up to 367,000 theoretical plates, reproducibility of corrected peak areas below 3% relative standard deviation, concentration detection limits from 0.04 to 1.3 μM and mass detection limits at sub femtomole levels (detection at 254 nm).

History

Publication title

The Analyst

Volume

123

Issue

12

Pagination

2865-2870

ISSN

0003-2654

Department/School

School of Natural Sciences

Publisher

RSC

Place of publication

Cambridge, UK

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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