The use of the gradient of the eluent flow rate is proposed for the optimization of separation in high-performance liquid chromatography (HPLC). This approach was used for the separation of the homologues of polycyclic aromatic compounds and di-n-alkyl phthalates on monolithic porous silica gel Chromolith (Merck, Germany) with modifying octadecyl radicals by reversed-phase HPLC. The principle of the optimization of the gradient of the eluent flow rate for the separation of the homologues of organic compounds was formulated on the basis of peculiarities in their chromatographic retention. It was demonstrated that using the gradient of the flow rate provides the selective and efficient separation of homologues and a significant decrease in the time of separation. © 2005 Pleiades Publishing, Inc.

Item Type:	Refereed Article
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Separation science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Nesterenko, PN (Professor Pavel Nesterenko)
ID Code:	47590
Year Published:	2005
Web of Science® Times Cited:	11
Deposited By:	Chemistry
Deposited On:	2007-09-18
Last Modified:	2009-11-13
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