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A method for forming internal pH gradients in chromatographic column filled with a carboxylic cationite

journal contribution
posted on 2023-05-16, 20:01 authored by Ivanov, AV, Vakshtein, MS, Nesterenko, PN, Khasanova, EM
Chromatofocusing was used to form descending pH gradients inside a column packed with a cationite based on supercrosslinked polystyrene with carboxy groups. The column was preliminary equilibrated with a starting buffer solution (10 mM Tris-HCl) to pH 7.5. It was demonstrated that pH gradients can be formed using buffer eluents containing a carboxylic acid or an amino acid as the active component. In the system under study, pH gradients with a high degree of linearity can be obtained when a strong electrolyte (NaCl) is added to the eluent and starting solution.

History

Publication title

Russian Journal of Physical Chemistry

Volume

77

Issue

12

Pagination

2023-2026

ISSN

0036-0244

Department/School

School of Natural Sciences

Publisher

MAIK Nauka - Interperiodica

Place of publication

Russian Federation

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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