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A method for forming internal pH gradients in chromatographic column filled with a carboxylic cationite
journal contribution
posted on 2023-05-16, 20:01 authored by Ivanov, AV, Vakshtein, MS, Nesterenko, PN, Khasanova, EMChromatofocusing was used to form descending pH gradients inside a column packed with a cationite based on supercrosslinked polystyrene with carboxy groups. The column was preliminary equilibrated with a starting buffer solution (10 mM Tris-HCl) to pH 7.5. It was demonstrated that pH gradients can be formed using buffer eluents containing a carboxylic acid or an amino acid as the active component. In the system under study, pH gradients with a high degree of linearity can be obtained when a strong electrolyte (NaCl) is added to the eluent and starting solution.
History
Publication title
Russian Journal of Physical ChemistryVolume
77Issue
12Pagination
2023-2026ISSN
0036-0244Department/School
School of Natural SciencesPublisher
MAIK Nauka - InterperiodicaPlace of publication
Russian FederationRepository Status
- Restricted