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A method for forming internal pH gradients in chromatographic column filled with a carboxylic cationite

Citation

Ivanov, AV and Vakshtein, MS and Nesterenko, PN and Khasanova, EM, A method for forming internal pH gradients in chromatographic column filled with a carboxylic cationite, Russian Journal of Physical Chemistry, 77, (12) pp. 2023-2026. ISSN 0036-0244 (2003) [Refereed Article]

Abstract

Chromatofocusing was used to form descending pH gradients inside a column packed with a cationite based on supercrosslinked polystyrene with carboxy groups. The column was preliminary equilibrated with a starting buffer solution (10 mM Tris-HCl) to pH 7.5. It was demonstrated that pH gradients can be formed using buffer eluents containing a carboxylic acid or an amino acid as the active component. In the system under study, pH gradients with a high degree of linearity can be obtained when a strong electrolyte (NaCl) is added to the eluent and starting solution.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Nesterenko, PN (Professor Pavel Nesterenko)
ID Code:47543
Year Published:2003
Web of Science® Times Cited:1
Deposited By:Chemistry
Deposited On:2007-09-18
Last Modified:2007-09-18
Downloads:0

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