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A method for forming internal pH gradients in chromatographic column filled with a carboxylic cationite
Citation
Ivanov, AV and Vakshtein, MS and Nesterenko, PN and Khasanova, EM, A method for forming internal pH gradients in chromatographic column filled with a carboxylic cationite, Russian Journal of Physical Chemistry, 77, (12) pp. 2023-2026. ISSN 0036-0244 (2003) [Refereed Article]
Abstract
Chromatofocusing was used to form descending pH gradients inside a column packed with a cationite based on supercrosslinked polystyrene with carboxy groups. The column was preliminary equilibrated with a starting buffer solution (10 mM Tris-HCl) to pH 7.5. It was demonstrated that pH gradients can be formed using buffer eluents containing a carboxylic acid or an amino acid as the active component. In the system under study, pH gradients with a high degree of linearity can be obtained when a strong electrolyte (NaCl) is added to the eluent and starting solution.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Chemical Sciences |
| Research Group: | Analytical Chemistry |
| Research Field: | Separation Science |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Chemical Sciences |
| UTAS Author: | Nesterenko, PN (Professor Pavel Nesterenko) |
| ID Code: | 47543 |
| Year Published: | 2003 |
| Web of Science® Times Cited: | 1 |
| Deposited By: | Chemistry |
| Deposited On: | 2007-09-18 |
| Last Modified: | 2007-09-18 |
| Downloads: | 0 |
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