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Formation of a descending pH gradient in a column filled with a carboxylic cation exchanger

journal contribution
posted on 2023-05-16, 20:01 authored by Ivanov, AV, Vakshtein, MS, Nesterenko, PN
Descending pH gradients in a colum filled with an SM-52 carboxylic cation-exchange sorbent based on cellulose were formed using the chromatofocusing technique. A 10 mM tris-HCl solution was used as the starting buffer solution, and a Polybuffer-96 solution (1:20), as the eluent forming the pH gradient. It was established that linear pH gradients in the cation-exchange system were obtainable due to the addition of a strong electrolyte (NaCl) to the starting solution and to the eluent.

History

Publication title

Russian Journal of Physical Chemistry

Volume

77

Pagination

131-133

ISSN

0036-0244

Department/School

School of Natural Sciences

Publisher

MAIK Nauka - Interperiodica

Place of publication

Russian Federation

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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