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Formation of a descending pH gradient in a column filled with a carboxylic cation exchanger
journal contribution
posted on 2023-05-16, 20:01 authored by Ivanov, AV, Vakshtein, MS, Nesterenko, PNDescending pH gradients in a colum filled with an SM-52 carboxylic cation-exchange sorbent based on cellulose were formed using the chromatofocusing technique. A 10 mM tris-HCl solution was used as the starting buffer solution, and a Polybuffer-96 solution (1:20), as the eluent forming the pH gradient. It was established that linear pH gradients in the cation-exchange system were obtainable due to the addition of a strong electrolyte (NaCl) to the starting solution and to the eluent.
History
Publication title
Russian Journal of Physical ChemistryVolume
77Pagination
131-133ISSN
0036-0244Department/School
School of Natural SciencesPublisher
MAIK Nauka - InterperiodicaPlace of publication
Russian FederationRepository Status
- Restricted