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Simultaneous ion chromatographic separation of anions and cations on poly(aspartic acid) functionalized silica
journal contribution
posted on 2023-05-16, 19:58 authored by Kiseleva, MG, Kebets, PA, Nesterenko, PNPoly(aspartic acid)-silica (PolyCAT A), originally designed for the cation-exchange chromatography of proteins, is proposed for the simultaneous ion chromatographic separation of inorganic anions and cations. This is possible owing to the zwitterion-exchange properties of this stationary phase, which are attributed to the presence of both protonated aminopropyl and dissociated carboxylic groups in poly(aspartic acid) attached to the silica. The retention of alkali metal (Li+, Na+, K+), alkaline earth metal (Mg2+, Ca2+), ammonium and inorganic anions (Cl-, H2PO4-, Br-, NO2-, I-, IO3-, NO3-, ClO4-, SCN-) was tested in aqueous solutions of sulfuric, perchloric, sulfosalicylic, citric, oxalic, maleic and aspartic acids with conductimetric detection. The effect of eluent pH, together with the concentration and characteristics of the eluting ions, were studied. Under optimum conditions (0.3 mmol dm-3 H2SO4-0.2 mmol dm-3 Li2SO4 eluent), the simultaneous separation of three anions (CI-, H2PO4-, NO3-) and four cations (Na+, K+, Mg2+, Ca2+), on a PolyCAT A column (200 x 4.6 mm id, 5 μm film thickness) was achieved in 9 min.
History
Publication title
The AnalystVolume
126Issue
12Pagination
2119-2123ISSN
0003-2654Department/School
School of Natural SciencesPublisher
Royal Society of ChemistryPlace of publication
United KingdomRepository Status
- Restricted