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Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR

Citation

Park, TG and Bell, EM and Pearce, I and Rublee, PA and Bolch, CJS and Hallegraeff, GM, Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR, Polar Biology, 30, (7) pp. 843-848. ISSN 0722-4060 (2007) [Refereed Article]

DOI: doi:10.1007/s00300-006-0244-0

Abstract

The heterotrophic dinoflagellate Pfiesteria piscicida was detected in Ace Lake in the Vestfold Hills, eastern Antarctica by using real-time PCR based on 18S rDNA sequences. Antarctic water samples collected in 2004 were tested by species-specific real-time PCR assays for the identification of P. piscicida and P. shumwayae. Positive results were shown with P. piscicida-specific real-time PCR, and PCR products were examined by sequence analysis for confirmation. A phylogenetic tree made from partial 18S rDNA sequences showed that the Antarctic clone clustered with P. piscicida. This result suggests that P. piscicida is present in the extreme conditions of an Antarctic saline lake which has not contained fish for thousands of years. © 2007 Springer-Verlag.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Phycology (incl. Marine Grasses)
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Antarctic and Sub-Antarctic Flora, Fauna and Biodiversity
Author:Park, TG (Mr Taie Park)
Author:Bolch, CJS (Dr Christopher Bolch)
Author:Hallegraeff, GM (Professor Gustaaf Hallegraeff)
ID Code:46709
Year Published:2007
Web of Science® Times Cited:9
Deposited By:Plant Science
Deposited On:2007-08-01
Last Modified:2008-05-26
Downloads:0

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