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Morphological and molecular genetic characterization of Cryptoperidiniopsis brodyi (Dinophyceae) from Australia-wide isolates

Citation

Park, TG and Bolch, CJS and Hallegraeff, GM, Morphological and molecular genetic characterization of Cryptoperidiniopsis brodyi (Dinophyceae) from Australia-wide isolates, Harmful Algae, 6, (5) pp. 718-733. ISSN 1568-9883 (2007) [Refereed Article]

DOI: doi:10.1016/i.hal.2007.02.004

Abstract

Cryptoperidiniopsis brodyi is a common heterotrophic dinoflagellate known to often co-occur with Pfiesteria species in eastern U.S. estuaries. In this study, C. brodyi from Australia and Pfiesteria piscicida from ballast water from Indonesia were characterized by morphological and genetic analyses. Two P. piscicida strains originating from ballast water samples showed little genetic differences compared to P. piscicida from other countries and their morphology was identical. This finding indicates a potential inflow of P. piscicida into Australian estuaries via ballast water. Nine cultures of C. brodyi were established from Tasmania, South Australia and Western Australia. All C. brodyi cultures exhibited identical thecal plate patterns and could not be discriminated from other non-Australian strains. In contrast, two distinct genotypes could be identified by rDNA sequence analyses which were distinct from the U.S. genotype of C. brodyi. A previous survey using PCR-based methods reported a wide distribution of Pfiesteria shumwayae in Australia. However, the present study demonstrated that SSU rDNA-based P. shumwayae-specific primers produce false-positive PCR reactions with Australian C. brodyi. These results suggest that genetic variants of C. brodyi are widely distributed in Australia and Australian genotypes of C. brodyi had previously been misidentified as P. shumwayae. This finding also indicates that previous Australian distribution studies of P. shumwayae using SSU rDNA-based primers are potentially erroneous and need to be revisited. © 2007 Elsevier B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Phycology (incl. Marine Grasses)
Objective Division:Environment
Objective Group:Air Quality
Objective Field:Marine Air Quality
Author:Park, TG (Mr Taie Park)
Author:Bolch, CJS (Dr Christopher Bolch)
Author:Hallegraeff, GM (Professor Gustaaf Hallegraeff)
ID Code:46665
Year Published:2007
Web of Science® Times Cited:6
Deposited By:Plant Science
Deposited On:2007-08-01
Last Modified:2008-06-03
Downloads:0

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