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A cost-effective method of preparing larval fish otoliths for reading using enzyme digestion and staining

Citation

Green, BS and Reilly, SM and McCormick, MI, A cost-effective method of preparing larval fish otoliths for reading using enzyme digestion and staining, Journal of Fish Biology, 61, (6) pp. 1600-1605. ISSN 0022-1112 (2002) [Refereed Article]

DOI: doi:10.1006/jfbi.2002.2177

Abstract

A fast and cost-effective method for examining otoliths in fish larvae was developed whereby the otolith remains in situ. Whole fish of the clownfish Amphiprion melanopus were enzyme-cleared using a laundry pre-soak and then stained using the Von Kossa silver staining method for calcium. The otolith nucleus, daily rings and the otolith edge were all clearly visible and were suitable for a variety of age and growth analyses. The total 'hands on' time required to process these otoliths was c. 3 min, and multiple samples could be processed simultaneously. The reduction in labour of this method to produce clear daily rings in the otolith lends itself to broad use in fish biology where large quantities of otoliths need to be examined in a cost- and time-efficient manner. © 2002 The Fisheries Society of the British Isles. Published by Elsevier Science Ltd. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal Structure and Function
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Green, BS (Associate Professor Bridget Green)
ID Code:44669
Year Published:2002
Web of Science® Times Cited:2
Deposited By:TAFI - Marine Research Laboratory
Deposited On:2007-06-14
Last Modified:2007-06-14
Downloads:0

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