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A cost-effective method of preparing larval fish otoliths for reading using enzyme digestion and staining

journal contribution
posted on 2023-05-16, 19:17 authored by Bridget Green, Reilly, SM, McCormick, MI
A fast and cost-effective method for examining otoliths in fish larvae was developed whereby the otolith remains in situ. Whole fish of the clownfish Amphiprion melanopus were enzyme-cleared using a laundry pre-soak and then stained using the Von Kossa silver staining method for calcium. The otolith nucleus, daily rings and the otolith edge were all clearly visible and were suitable for a variety of age and growth analyses. The total 'hands on' time required to process these otoliths was c. 3 min, and multiple samples could be processed simultaneously. The reduction in labour of this method to produce clear daily rings in the otolith lends itself to broad use in fish biology where large quantities of otoliths need to be examined in a cost- and time-efficient manner. © 2002 The Fisheries Society of the British Isles. Published by Elsevier Science Ltd. All rights reserved.

History

Publication title

Journal of Fish Biology

Volume

61

Issue

6

Pagination

1600-1605

ISSN

0022-1112

Department/School

Institute for Marine and Antarctic Studies

Publisher

Academic Press Ltd Elsevier Science

Place of publication

England

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the environmental sciences

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