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Environmental factors affecting transcription of the human L1 Hs retrotransposon: 2. Stressors


Morales, JF and Snow, ET and Murnane, J, Environmental factors affecting transcription of the human L1 Hs retrotransposon: 2. Stressors, Mutagenesis, 18, (2) pp. 151-158. ISSN 0267-8357 (2003) [Refereed Article]

DOI: doi:10.1093/mutage/18.2.151


Retrotransposons have clearly molded the structure of the human genome. The reverse transcriptase coded for by long interspersed nuclear elements (LINEs) accounts for 35% of the human genome, with 8-9 × 105 copies of the most common human LINE element, L1Hs. Retrotransposons cycle through an RNA intermediate with transcription as the rate limiting step. Because various retrotransposons have been demonstrated to be induced by environmental stimuli, we investigated the response of the L1Hs promoter to various agents. L1Hs promoter activity was analyzed by transfecting an L1Hs-expressing cell line with plasmids containing one of two L1Hs promoters fused to the LacZ reporter gene. L1Hs promoter activity was then monitored with a β-galactosidase assay. Treatment with UV light and heat shock resulted in a small increase in β-galactosidase activity from one promoter, while treatment with tetradecanoylphorbol 13-acetate resulted in small increases in β-galactosidase activity from both promoters. No increase in β-galactosidase activity was observed after exposure to X-rays or hydrogen peroxide.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Pharmacology and pharmaceutical sciences
Research Field:Toxicology (incl. clinical toxicology)
Objective Division:Health
Objective Group:Public health (excl. specific population health)
Objective Field:Public health (excl. specific population health) not elsewhere classified
UTAS Author:Snow, ET (Associate Professor Elizabeth Snow)
ID Code:44628
Year Published:2003
Web of Science® Times Cited:25
Deposited By:Health Sciences A
Deposited On:2007-10-19
Last Modified:2007-10-19

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