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Further insight into the Heparin-Releasable and Glycosylphosphatidylinositol-lipid anchored forms of tissue factor pathway inhibitor

Citation

Ellery, PER and Hardy, K and Oostryck, R and Adams, MJ, Further insight into the Heparin-Releasable and Glycosylphosphatidylinositol-lipid anchored forms of tissue factor pathway inhibitor, Clinical and Applied Thrombosis and Haemostasis, 14, (3) pp. 267-278. ISSN 1076-0296 (2008) [Refereed Article]


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Copyright Statement

Copyright 2008, Clinical and Applied Thrombosis/Hemostasis Online by SAGE Publications

Official URL: http://cat.sagepub.com

DOI: doi:10.1177/1076029607304239

Abstract

The release of tissue factor pathway inhibitor (TFPI) from human umbilical vein endothelial cells (HUVECs) was investigated using heparin and phospholipase C. The experiment included incubating HUVECs with 0, 1, or 10 U/mL heparin diluted in Dulbecco Modified Eagle's Medium plus 5% fetal calf serum for 1 or 24 hours. A statistically significant increase in TFPI activity levels was seen at 1 hour, but not at 24 hours. A 20-fold increase in the release of TFPI after phospholipase C treatment of HUVECs was demonstrated, confirming that it is glycosylphosphatidylinositol-lipid (GPI) anchored. Sequential treatment of HUVECs with phospholipase C and heparin was performed, and a trend was observed where GPI-anchored TFPI levels were increased after 1 hour of pretreatment with heparin but were decreased after 24 hours. Serum is a requirement for the heparin-dependent release of TFPI from HUVECs. Heparin pretreatment of HUVECs may affect levels of GPI anchored TFPI in a time and dose-dependent manner.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Cardiorespiratory Medicine and Haematology
Research Field:Haematology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Blood Disorders
Author:Adams, MJ (Dr Murray Adams)
ID Code:44574
Year Published:2008
Web of Science® Times Cited:6
Deposited By:Health Sciences A
Deposited On:2008-07-03
Last Modified:2009-06-01
Downloads:1 View Download Statistics

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