University of Tasmania
Browse

File(s) under permanent embargo

Further insight into the Heparin-Releasable and Glycosylphosphatidylinositol-lipid anchored forms of tissue factor pathway inhibitor

journal contribution
posted on 2023-05-16, 19:15 authored by Ellery, PER, Hardy, K, Oostryck, R, Adams, MJ
The release of tissue factor pathway inhibitor (TFPI) from human umbilical vein endothelial cells (HUVECs) was investigated using heparin and phospholipase C. The experiment included incubating HUVECs with 0, 1, or 10 U/mL heparin diluted in Dulbecco Modified Eagle's Medium plus 5% fetal calf serum for 1 or 24 hours. A statistically significant increase in TFPI activity levels was seen at 1 hour, but not at 24 hours. A 20-fold increase in the release of TFPI after phospholipase C treatment of HUVECs was demonstrated, confirming that it is glycosylphosphatidylinositol-lipid (GPI) anchored. Sequential treatment of HUVECs with phospholipase C and heparin was performed, and a trend was observed where GPI-anchored TFPI levels were increased after 1 hour of pretreatment with heparin but were decreased after 24 hours. Serum is a requirement for the heparin-dependent release of TFPI from HUVECs. Heparin pretreatment of HUVECs may affect levels of GPI anchored TFPI in a time and dose-dependent manner.

History

Publication title

Clinical and Applied Thrombosis and Haemostasis

Volume

14

Pagination

267-278

ISSN

1076-0296

Department/School

School of Health Sciences

Publisher

Sage Publications

Place of publication

USA

Rights statement

Copyright © 2008, Clinical and Applied Thrombosis/Hemostasis Online by SAGE Publications

Repository Status

  • Restricted

Socio-economic Objectives

Diagnosis of human diseases and conditions

Usage metrics

    University Of Tasmania

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC