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Retention volume of acidic compounds in ion exclusion chromatography
Citation
Glod, BK and Haddad, PR and Alexander, PW, Retention volume of acidic compounds in ion exclusion chromatography, Acta Chromatographica, 3 pp. 23-26. ISSN 1233-2356 (1994) [Refereed Article]
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Copyright Statement
© 1994 Akademiai Kiado Rt.
Official URL: http://www.akademiai.com
Abstract
The separation mechanism of acidic compounds in Ion-Exclusion
Chromatography is described assuming that the stationary phase contains
immobilised ions (remnants of the' dissociated functional groups) that
cannot be transferred to the mobile phase. Other properties of the two
chromatographic phases are assumed to be the same. System description is
based on computer simulation of column performance by the Craig (finite
element) method. The thermodynamic equilibria conditions are applied
locally to a small but finite element of the column, corresponding to a
theoretical plate, in a recurrent procedure to simulate the passage ofthe
sample down the column. Partition of the analysed sample between the
stationary and the mobile phases is calculated for every plate. The portion
of the mobile phase is then moved to the next plate, the process being
continued repeatedly. This procedure makes it possible to study the
chromatographic peak evolution with time and also the influence of the
parameters characterising the chromatographic column, the mobile phase
and the analysed sample, on retention volume and peak shape of the
sample.
Calculations of retention volume as a function of acidic dissociation
constant, sample injection quantity and buffer concentration were
performed for pure water and aqueous buffer as the mobile phases. Results
are in good agreement with experimental data. It was found that the buffer
eliminates peak leading and retention volume dependence on sample
concentration. It was also ascertained. that, as in pure water, strong acids
are eluted together at the start of the chromatogram and are not retained on
the column. Weak acids are also eluted together, at the end of the
chromatogram.
Item Details
Item Type: | Refereed Article |
---|---|
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Instrumental methods (excl. immunological and bioassay methods) |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Haddad, PR (Professor Paul Haddad) |
UTAS Author: | Alexander, PW (Professor Peter Alexander) |
ID Code: | 4428 |
Year Published: | 1994 |
Deposited By: | Applied Science |
Deposited On: | 1995-08-01 |
Last Modified: | 2011-09-19 |
Downloads: | 2 View Download Statistics |
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