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High-resolution mapping of the Alp locus and identification of a candidate gene HvMate controlling aluminium tolerancein barley (Hordeum vulgare L.)
Citation
Wang, Junping and Raman, H and Zhou, M and Ryan, PR and Delhaize, E and Hebb, DM and Coombes, N and Mendham, NJ, High-resolution mapping of the Alp locus and identification of a candidate gene HvMate controlling aluminium tolerancein barley (Hordeum vulgare L.) , Theoretical and Applied Genetics, 115, (2) pp. 265-276. ISSN 0040-5752 (2007) [Refereed Article]
DOI: doi:10.1007/s00122-007-0562-9
Abstract
Aluminium (Al) tolerance in barley is conditioned by the Alp locus on the long arm of chromosome 4H, which is associated with Al-activated release of citrate from roots. We developed a high-resolution map of the Alp locus using 132 doubled haploid (DH) lines from a cross between Dayton (Al-tolerant) and Zhepi 2 (Al-sensitive) and 2,070 F2 individuals from a cross between Dayton and Gairdner (Al-sensitive). The Al-activated efflux of citrate from the root apices of Al-tolerant Dayton was 10-fold greater than from the Al-sensitive parents Zhepi 2 and Gairdner. A suite of markers (ABG715, Bmag353, GBM1071, GWM165, HvMATE and HvGABP) exhibited complete linkage with the Alp locus in the DH population accounting 72% of the variation for Al tolerance evaluated as relative root elongation. These markers were used to map this genomic region in the Dayton/Gairdner population in more detail. Flanking markers HvGABP and ABG715 delineated the Alp locus to a 0.2 cM interval. Since the HvMATE marker was not polymorphic in the Dayton/Gairdner population we instead investigated the expression of the HvMATE gene. Relative expression of the HvMATE gene was 30-fold greater in Dayton than Gardiner. Furthermore, HvMATE expression in the F2:3 families tested, including all the informative recombinant lines identified between HvGABP and ABG715 was significantly correlated with Al tolerance and Al-activated citrate efflux. These results identify HvMATE, a gene encoding a multidrug and toxic compound extrusion protein, as a candidate controlling Al tolerance in barley. © 2007 Springer-Verlag.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Agricultural, Veterinary and Food Sciences |
| Research Group: | Crop and pasture production |
| Research Field: | Crop and pasture improvement (incl. selection and breeding) |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Grains and seeds |
| Objective Field: | Barley |
| UTAS Author: | Wang, Junping (Ms Junping Wang) |
| UTAS Author: | Zhou, M (Professor Meixue Zhou) |
| UTAS Author: | Mendham, NJ (Dr Neville Mendham) |
| ID Code: | 44067 |
| Year Published: | 2007 |
| Web of Science® Times Cited: | 25 |
| Deposited By: | Agricultural Science |
| Deposited On: | 2007-08-01 |
| Last Modified: | 2009-08-06 |
| Downloads: | 0 |
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