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High-resolution mapping of the Alp locus and identification of a candidate gene HvMate controlling aluminium tolerancein barley (Hordeum vulgare L.)

Citation

Wang, Junping and Raman, H and Zhou, M and Ryan, PR and Delhaize, E and Hebb, DM and Coombes, N and Mendham, NJ, High-resolution mapping of the Alp locus and identification of a candidate gene HvMate controlling aluminium tolerancein barley (Hordeum vulgare L.) , Theoretical and Applied Genetics, 115, (2) pp. 265-276. ISSN 0040-5752 (2007) [Refereed Article]

DOI: doi:10.1007/s00122-007-0562-9

Abstract

Aluminium (Al) tolerance in barley is conditioned by the Alp locus on the long arm of chromosome 4H, which is associated with Al-activated release of citrate from roots. We developed a high-resolution map of the Alp locus using 132 doubled haploid (DH) lines from a cross between Dayton (Al-tolerant) and Zhepi 2 (Al-sensitive) and 2,070 F2 individuals from a cross between Dayton and Gairdner (Al-sensitive). The Al-activated efflux of citrate from the root apices of Al-tolerant Dayton was 10-fold greater than from the Al-sensitive parents Zhepi 2 and Gairdner. A suite of markers (ABG715, Bmag353, GBM1071, GWM165, HvMATE and HvGABP) exhibited complete linkage with the Alp locus in the DH population accounting 72% of the variation for Al tolerance evaluated as relative root elongation. These markers were used to map this genomic region in the Dayton/Gairdner population in more detail. Flanking markers HvGABP and ABG715 delineated the Alp locus to a 0.2 cM interval. Since the HvMATE marker was not polymorphic in the Dayton/Gairdner population we instead investigated the expression of the HvMATE gene. Relative expression of the HvMATE gene was 30-fold greater in Dayton than Gardiner. Furthermore, HvMATE expression in the F2:3 families tested, including all the informative recombinant lines identified between HvGABP and ABG715 was significantly correlated with Al tolerance and Al-activated citrate efflux. These results identify HvMATE, a gene encoding a multidrug and toxic compound extrusion protein, as a candidate controlling Al tolerance in barley. © 2007 Springer-Verlag.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Improvement (Selection and Breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
Author:Wang, Junping (Ms Junping Wang)
Author:Zhou, M (Associate Professor Meixue Zhou)
Author:Mendham, NJ (Dr Neville Mendham)
ID Code:44067
Year Published:2007
Web of Science® Times Cited:70
Deposited By:Agricultural Science
Deposited On:2007-08-01
Last Modified:2009-08-06
Downloads:0

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