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Considerations on contactless conductivity detection in capillary electrophoresis

journal contribution
posted on 2023-05-16, 18:59 authored by Baltussen, E, Rosanne Guijt, van der Steen, G, Laugere, F, Baltussen, S, van Dedem, GWK
Nearly all analyses by capillary electrophoresis (CE) are performed using optical detection, utilizing either absorbance or (laser-induced) fluorescence. Though adequate for many analytical problems, in a large number of cases, e.g., involving non-UV-absorbing compounds, these optical detection methods fall short. Indirect optical detection can then still provide an acceptable means of detection, however, with a strongly reduced sensitivity. During the past few years, contactless conductivity detection (CCD) has been presented as a valuable extension to optical detection techniques. It has been demonstrated that with CCD detection limits comparable, or even superior, to (indirect) optical detection can be obtained. Additionally, construction of the CCD around the CE capillary is straightforward and robust operation is easily obtained. Unfortunately, in the literature a large variety of designs and operating conditions for CCD were described. In this contribution, several important parameters of CCD are identified and their influence on, e.g., detectability and peak shape is described. An optimized setup based on a well-defined detection cell with three detection electrodes is presented. Additionally, simple and commercially available read-out electronics are described. The performance of the CCD-CE system was demonstrated for the analysis of peptides. Detection limits at the M level were obtained in combination with good peak shapes and an overall good performance and stability.

History

Publication title

Electrophoresis

Volume

23

Issue

17

Pagination

2888-2893

ISSN

0173-0835

Department/School

School of Natural Sciences

Publisher

Wiley

Place of publication

Weinheim

Rights statement

Copyright 2002 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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