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In this contribution, the role of bioaffinity interactions on electrokinetically controlled microfabricated devices is reviewed. Interesting applications reported in the literature include enzymatic assays, where enzyme and enzyme inhibition kinetics were studied, often in combination with electrophoretic separation. Attention is paid towards developments that could lead to implementation of electrokinetically controlled microdevices in high-throughput screening. Furthermore, enzyme-facilitated detection in combination with electrophoretic separation on microdevices is discussed. Various types of immunoassays have been implemented on the microchip format. The selectivity of antibody-antigen interaction has been exploited for the detection of analytes in complex sample matrices as required, for example, in clinical chemistry. Binding kinetics as well as stoichiometry were studied in chip-based assays. Automated mixing protocols as well as the demonstration of a parallel immunoassay allow implementation of microdevices in high-throughput screening. Furthermore, demonstration of immunoassays on cheap polymeric microdevices opens the way towards the fabrication of disposable devices, a requirement for commercialization and therefore for application in routine analyses.

Item Type:	Refereed Article
Keywords:	Affinity interactions; Binding constants; Electrophoresis; Molecular recognition; Review; -Total analysis system
Research Division:	Biological Sciences
Research Group:	Biochemistry and cell biology
Research Field:	Analytical biochemistry
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Guijt, RM (Dr Rosanne Guijt)
ID Code:	43561
Year Published:	2002
Web of Science® Times Cited:	32
Deposited By:	Chemistry
Deposited On:	2007-03-15
Last Modified:	2010-03-15
Downloads:	2 View Download Statistics