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Recent advances in affinity capillary electrophoresis


Guijt, RM and Frank, J and van Dedem, GWK and Baltussen, E, Recent advances in affinity capillary electrophoresis, Electrophoresis, 21, (18) pp. 3905-3918. ISSN 0173-0835 (2000) [Refereed Article]

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DOI: doi:10.1002/1522-2683(200012)21:18<3905::AID-ELPS3905>3.0.CO;2-4


Use of the specificity of (bio)interactions can effectively overcome the selectivity limitation faced in capillary electrophoresis (CE), and the resulting technique usually is referred to as affinity capillary electrophoresis (ACE). Despite the high selectivity of ACE, several important problems still need to be addressed. A major issue in all CE separations, including ACE, is the concentration detection limit. Using UV detection, this is usually in the order of 10 - 6 M whereas laser-induced fluorescence (LIF) detection can provide detection limits down to the sub-10 - 10 M range. However, a marked disadvantage of LIF is that labeling of the analytes is usually required, which might change the interaction behavior of the solutes under investigation. Additionally, labeling reactions at sub-10 - 10 M concentration levels are certainly not trivial and often difficult to perform quantitatively. Alternative and universal detection approaches, particularly mass spectrometric (MS) detection, look very promising but (A) CE-MS techniques are still far from routine application. Important future progress in sensitive detection strategies is likely to increase the use of ACE in the future.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Analytical biochemistry
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Guijt, RM (Dr Rosanne Guijt)
ID Code:43559
Year Published:2000
Web of Science® Times Cited:71
Deposited By:Chemistry
Deposited On:2007-03-15
Last Modified:2010-03-01
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