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Towards a microchip-based chromatographic platform. Part 2: Sol-gel phases modified with polyelectrolyte multilayers for capillary electrochromatography

journal contribution
posted on 2023-05-16, 18:58 authored by Michael BreadmoreMichael Breadmore, Shrinivasan, S, Ferrance, JP, Power, ME, Norris, PM, Landers, JP, Karlinsey, J
The potential for using polyelectrolyte multilayers (PEMs) to provide chromatographic functionality on continuous silica networks created from sol-gel chemistry has been evaluated by capillary electrochromatography (CEC). Construction of the PEM was achieved by flushing the column with polyelectrolytes of alternative charge, with variation of the properties of the exposed polyelectrolyte providing a unique means to vary the chromatographic surface. Variation of the exposed polyelectrolyte from poly(diallyldimethylammonium chloride) (PDDAC) to dextran sulfate (DS) allowed the direction of the electroosmotic flow (EOF) to be changed and also provided a means to vary the chromatographic capacity. Variation of negative polymer from DS to poly(styrene sulfonate) (PSS) significantly altered the EOF and the migration of peptides, with both the reversed-phase and ion-exchange capacities increasing. An alternative method for changing the column capacity was to change the thickness of the PEM, which was evaluated by anion-exchange CEC. A 70-80% increase in retention was observed for all anions without any increase in EOF suggesting significant penetration of the analytes through the PEM and interaction with buried charges within the PEM.

History

Publication title

Electrophoresis

Volume

24

Issue

7-8

Pagination

1261-1270

ISSN

0173-0835

Department/School

School of Natural Sciences

Publisher

Wiley

Place of publication

Weinheim

Rights statement

The original publication is available at http://www.interscience.wiley.com

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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