eCite Digital Repository

Towards a microchip-based chromatographic platform. Part 2: Sol-gel phases modified with polyelectrolyte multilayers for capillary electrochromatography

Citation

Breadmore, MC and Shrinivasan, S and Ferrance, JP and Power, ME and Norris, PM and Landers, JP and Karlinsey, J, Towards a microchip-based chromatographic platform. Part 2: Sol-gel phases modified with polyelectrolyte multilayers for capillary electrochromatography, Electrophoresis, 24, (7-8) pp. 1261-1270. ISSN 0173-0835 (2003) [Refereed Article]


Preview
PDF
Restricted - Request a copy
181Kb
  

Copyright Statement

The original publication is available at http://www.interscience.wiley.com

Official URL: http://www.interscience.wiley.com

DOI: doi:10.1002/elps.200390162

Abstract

The potential for using polyelectrolyte multilayers (PEMs) to provide chromatographic functionality on continuous silica networks created from sol-gel chemistry has been evaluated by capillary electrochromatography (CEC). Construction of the PEM was achieved by flushing the column with polyelectrolytes of alternative charge, with variation of the properties of the exposed polyelectrolyte providing a unique means to vary the chromatographic surface. Variation of the exposed polyelectrolyte from poly(diallyldimethylammonium chloride) (PDDAC) to dextran sulfate (DS) allowed the direction of the electroosmotic flow (EOF) to be changed and also provided a means to vary the chromatographic capacity. Variation of negative polymer from DS to poly(styrene sulfonate) (PSS) significantly altered the EOF and the migration of peptides, with both the reversed-phase and ion-exchange capacities increasing. An alternative method for changing the column capacity was to change the thickness of the PEM, which was evaluated by anion-exchange CEC. A 70-80% increase in retention was observed for all anions without any increase in EOF suggesting significant penetration of the analytes through the PEM and interaction with buried charges within the PEM.

Item Details

Item Type:Refereed Article
Keywords:Capillary electrochromatography Miniaturization Polyelectrolyte multilayers Silica monolithic column Sol-gel phase
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:43547
Year Published:2003
Web of Science® Times Cited:19
Deposited By:Chemistry
Deposited On:2007-03-15
Last Modified:2010-03-14
Downloads:0

Repository Staff Only: item control page