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Non-aqueous capillary electrophoresis with red light emitting diode absorbance detection for the analysis of basic dyes

Citation

Fakhari, AR and Breadmore, MC and Macka, M and Haddad, PR, Non-aqueous capillary electrophoresis with red light emitting diode absorbance detection for the analysis of basic dyes, Analytica Chimica Acta, 580, (2) pp. 188-193. ISSN 0003-2670 (2006) [Refereed Article]

DOI: doi:10.1016/j.aca.2006.07.066

Abstract

Non-aqueous capillary electrophoresis was evaluated for the separation of five hydrophobic basic blue dyes for application in forensic dye analysis. The use of a red light emitting diode as a high intensity, low-noise light source provided sensitive detection of the blue dyes while also allowing the evaluation of solvents that absorb strongly in the UV region. Excellent peak shapes and separation selectivity were obtained in methanol, ethanol, acetonitrile and dimethylsulfoxide, however water, tetrahydrofuran, dimethylformamide and acetone were unsuitable as solvents due to poor peak shapes and a lack of sensitivity, most likely due to adsorption onto the capillary wall. Due to the known compatibility of methanol with capillary electrophoresis-mass spectrometry, this solvent was examined further with the relative acidity/basicity of the electrolyte being optimised with an artificial neural network. The optimised method was examined for the separation of ink samples from 6 fibre tip and 2 ball point blue or black pens and showed that a unique migration time for the main dye component in seven of the eight pens could be obtained. © 2006 Elsevier B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Breadmore, MC (Professor Michael Breadmore)
Author:Macka, M (Professor Mirek Macka)
Author:Haddad, PR (Professor Paul Haddad)
ID Code:43032
Year Published:2006
Web of Science® Times Cited:32
Deposited By:Chemistry
Deposited On:2006-08-01
Last Modified:2009-11-18
Downloads:0

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