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Analyte-stationary phase interactions in ion-exclusion chromatography


Novic, M and Haddad, PR, Analyte-stationary phase interactions in ion-exclusion chromatography, Journal of Chromatography A, 1118, (1) pp. 19-28. ISSN 0021-9673 (2006) [Refereed Article]

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DOI: doi:10.1016/j.chroma.2006.02.090


The currently accepted analyte-stationary phase interactions occurring in ion-exclusion chromatography are re-examined. In particular, the requirement for the existence of a Donnan membrane separating the flowing, interstitial eluent from the static, occluded, liquid acting as the stationary phase is scrutinized, together with the role of hydrophobic adsorption effects in the retention of aromatic analytes. Plots showing the interconversion of the column between the analyte and eluent forms are used to highlight some shortcomings of the currently accepted mechanism for ion-exclusion chromatography. An alternative retention mechanism for ion-exclusion chromatography is proposed, based on the presence of a potential well at the surface of the fully functionalized styrene-divinylbenzene co-polymer stationary phase. Analytes diffuse into the potential well under the effects of concentration gradients, and undergo repulsion effects from the fixed charges inside the pores. The net contributions of these two opposing processes determine the degree to which an analyte is retained on the stationary phase. Negligible hydrophobic adsorption of the analyte onto the polymeric resin supporting the stationary phase is considered to occur.

Item Details

Item Type:Refereed Article
Keywords:Ion exclusion chromatography; Retention mechanism; Analyte-stationary phase interactions
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Novic, M (Dr Milko Novic)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:43027
Year Published:2006
Web of Science® Times Cited:25
Deposited By:Chemistry
Deposited On:2006-08-01
Last Modified:2010-02-01

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