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Sensitive determination of carbohydrates labelled with p-nitroaniline by capillary electrophoresis with photometric detection using a 406 nm light-emitting diode

Citation

Momenbeik, F and Johns, CA and Breadmore, MC and Hilder, EF and Macka, M and Haddad, PR, Sensitive determination of carbohydrates labelled with p-nitroaniline by capillary electrophoresis with photometric detection using a 406 nm light-emitting diode, Electrophoresis, 27, (20) pp. 4039-4046. ISSN 0173-0835 (2006) [Refereed Article]

DOI: doi:10.1002/elps.200500856

Abstract

p-Nitroaniline was explored s a derivatising reagent for UV absorbance detection of carbohydrates after separation by CE. This derivatising agent has three advantages: first, it has excellent water solubility; second, it has high molar absorptivity; and third, it is possible to obtain sensitive detection using a UV or blue light-emitting diode (LED) as the light source. The labelling reaction took less than 30 min to complete with high reaction yield. The separation process was modelled and optimised using an artificial neural network. Nine carbohydrates were separated by a CE system within 16 min using a 0.17 M boric acid buffer at pH 9.7. On-column LED detection at 406 nm allowed the detection of carbohydrates with good detection limits (<1.1μM or 8.8 fmol) and reproducible quanitification in the concentration range of 2.6-200 μM. This method was applied successfully to the determination of component carbohydrates in some food samples. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinhelm.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Momenbeik, F (Mr Fariborz Momenbeik)
Author:Johns, CA (Dr Cameron Johns)
Author:Breadmore, MC (Professor Michael Breadmore)
Author:Hilder, EF (Professor Emily Hilder)
Author:Macka, M (Professor Mirek Macka)
Author:Haddad, PR (Professor Paul Haddad)
ID Code:43026
Year Published:2006
Web of Science® Times Cited:16
Deposited By:Chemistry
Deposited On:2006-08-01
Last Modified:2009-11-18
Downloads:0

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