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Multiple shoot formation from mature seeds in lentils (Lens culinaris) G


Ye, G and McNeil, DL and Conner, AJ and Hill, GD, Multiple shoot formation from mature seeds in lentils (Lens culinaris) G, New Zealand Journal of Crop and Horticultural Science, 30 pp. 1-8. ISSN 0114-0671 (2002) [Refereed Article]

DOI: doi:10.1080/01140671.2002.9514193


A protocol based on seed culture was developed for efficient in vitro propagation of lentil (Lens culinaris Medik). Benzyladenine (BA), thidiazuron (TDZ), and kinetin all induced multiple shoot formation. In terms of the number of long shoots ( > 2.0 cm) produced per seed, BA and TDZ at optimum concentrations (0.2"0.4 and 0.1 mg/litre, respectively) had similar efficiency, whereas kinetin produced less shoots. Murashige and Skoog (MS) salt composition was better than that of Gamborge (B5) for shoot induction. Increasing calcium (Ca) concentration was necessary to overcome shoot-tip necrosis. For shoot elongation, fresh medium of the same composition of shoot induction medium could be used for stumps from medium with low BA ( < 0.8 mg/litre) or TDZ ( < 0.4 mg/litre). Otherwise, MS medium supplemented with 0.5 mg/ litre gibberellic acid (GA 3 ) or 0.05 mg/litre α-naphthaleneacetic acid (NAA) was appropriate. Multiple shoots were induced from 10 inbred of four species. Best rooting was achieved using MS medium supplemented with 1.5 mg/litre NAA. Using this rooting medium more than 50% shoots from the 10 inbreds rooted. From 80 to 100% plantlets of the 10 inbreds survived after being transferred to potting mixture in glass house. © 2002 Taylor & Francis Group, LLC.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture improvement (incl. selection and breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Grain legumes
UTAS Author:McNeil, DL (Professor David McNeil)
ID Code:42525
Year Published:2002
Web of Science® Times Cited:9
Deposited By:Agricultural Science
Deposited On:2007-02-22
Last Modified:2007-02-22

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