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In vitro effect of vitellogenin on steroid production by ovarian follicles of greenback flounder Rhombosolea tapirina


Sun, B and Pankhurst, NW, In vitro effect of vitellogenin on steroid production by ovarian follicles of greenback flounder Rhombosolea tapirina, Comparative Biochemistry and Physiology. Part A: Molecular and Integrative Physiology, 144, (1) pp. 78-85. ISSN 1095-6433 (2006) [Refereed Article]

DOI: doi:10.1016/j.cbpa.2006.02.007


Ovarian follicles from vitellogenic greenback flounder (Rhombosolea tapirina) were incubated in L15 medium alone, or containing human chorionic gonadotropin (hCG), dibutyryl cyclic AMP (dbcAMP) or the steroid precursors testosterone (T), 17-hydroxyprogesterone (17P) and androstenedione (A) in the presence of vitellogenin (Vtg) at 0.1-5.0 mg mL - 1. Medium concentrations of 17β-estradiol (E 2) and T were measured by radioimmunoassay. HCG generally stimulated follicular E 2 but not T production, whereas 17P, A and T stimulated production of E 2, T, and E 2 respectively. Treatment of follicles with dbcAMP inhibited follicular E 2 production, but increased follicular T production at high doses. The effect of low concentrations of Vtg on follicular steroid production was variable; however, higher doses of Vtg significantly suppressed basal, hCG-, dbcAMP- and steroid precursor-stimulated follicular E 2 and T production. The results of this study show that high concentrations of Vtg may suppress follicular steroid production by interfering in the steroidogenic pathway. This suggests that Vtg may regulate its own production by limiting the ovarian production of E 2. © 2006 Elsevier Inc. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Fisheries - aquaculture not elsewhere classified
UTAS Author:Sun, B (Mr Baoguo Sun)
ID Code:42025
Year Published:2006
Web of Science® Times Cited:5
Deposited By:TAFI - Aquaculture
Deposited On:2006-08-01
Last Modified:2011-09-30

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