Effects of aromatase inhibitors on in vitro steroidogenesis by Atlantic salmon (Salmo salar) gonadal and brain tissue

In order to assess the efficacy of selected aromatase inhibitors on Atlantic salmon (Salmo salar) ovarian and brain tissue, in vitro systems were developed for measuring 17β-estradiol (E 2) production by these tissues. Isolated vitellogenic follicles, or homogenised whole brains were incubated at 10 °C in complete Cortlands solution for 18 or 42 h respectively, and E 2 levels in the medium were determined by RIA. The addition of testosterone to the medium increased E 2 production in all preparations. E 2 production by whole brain homogenate was reduced by co-incubation with the aromatase inhibitors 1,4,6-androstatriene-3,17-dione (ATD), 4-androstene-4-ol-3,17-dione (OHA), aminoglutethimide, fadrozole or miconazole. Fadrozole, ATD, and OHA reduced E 2 production by vitellogenic follicles at a medium concentration of 0.1 μg mL - 1, whereas miconazole was only effective at 10 μg mL - 1. This study demonstrates a simple and rapid screening method for assessing the efficacy of aromatase inhibitors on fish tissues, and that the aromatase inhibitors ATD, OHA and fadrozole are potent inhibitors of both brain and gonadal aromatase in vitro, in Atlantic salmon. © 2006 Elsevier Inc. All rights reserved.