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Phenotypic and genotypic characterization of human and nonhuman Escherichia coli


Parveen, S and Hodge, NC and Stall, RE and Farrah, SR and Tamplin, ML, Phenotypic and genotypic characterization of human and nonhuman Escherichia coli, Water Research, 35 pp. 379-386. ISSN 0043-1354 (2001) [Refereed Article]

DOI: doi:10.1016/S0043-1354(00)00269-4


Estuarine waters receive fecal pollution from a variety of sources, including humans and wildlife. Escherichia coli is one of several fecal coliform bacteria that inhabit the intestines of many warm-blooded animals that sometime contaminate water. Its presence does not specifically implicate human fecal input, therefore it is necessary to differentiate contamination sources to accurately assess health risks. E. coli were isolated from human sources (HS) and nonhuman sources (NHS) in the Apalachicola National Estuarine Research Reserve and analyzed for fatty acid methyl ester (FAME), O-serogroup, and pulsed-field gel electrophoresis (PFGE) profiles. For FAME and PFGE analyses, there was no relationship between profile and isolate source. Human source PFGE profiles were less diverse than NHS isolates, and conversely for FAME. In contrast, O-serogrouping showed less diversity for HS vs. NHS isolates, and the predominant HS O-serogroups differed significantly (P < 0.01) from those of NHS isolates. (C) 2000 Elsevier Science Ltd.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Health
Objective Group:Public health (excl. specific population health)
Objective Field:Food safety
UTAS Author:Tamplin, ML (Professor Mark Tamplin)
ID Code:41194
Year Published:2001
Web of Science® Times Cited:49
Deposited By:Agricultural Science
Deposited On:2006-08-01
Last Modified:2006-12-13

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