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Plasma glutathione peroxidase by ELISA and relationship to selenium level


Jacobson, GA and Narkowicz, CK and Tong, YC and Peterson, GM, Plasma glutathione peroxidase by ELISA and relationship to selenium level, Clinica Chimica Acta, 369, (1) pp. 100-103. ISSN 0009-8981 (2006) [Refereed Article]

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DOI: doi:10.1016/j.cca.2006.01.007


Background Many assays have investigated GPx activity but there are few reports of immunoreactive methods of GPx plasma determinations and their relationship with plasma selenium. Methods A cross-sectional analysis of selenium and GPx was carried out on 170 adults randomly selected from the electoral role. Blood samples were collected and analysed for plasma GPx using a commercial enzyme-linked immunosorbent assay (ELISA) kit and plasma selenium using magnetic sector ICPMS. Further investigations of GPx level were carried out using one-dimensional (1D) and two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE). Results Plasma GPx was associated with plasma Se level (r2 = 0.24, P < 0.0001). Mean (range) Se was 108.9 (67.4268.0) g/l. Mean (range) plasma GPx was 18.5 (3.843.8) g/ml, significantly higher than the theoretical mean GPx of around 6.56.9 g/ml based on ICPMS results and the proportion of Se in intact GPx. GPx was higher in males than females and lower in current than non-smokers and ex-smokers. PAGE analysis of glycine dissociated ELISA wells revealed GPx as well as a number of unknown proteins with significant variability between high and low GPx samples. Conclusions Plasma GPx is correlated with Se status, however, there is considerable GPx variability between patients and ELISA methods appear to significantly overestimate true plasma GPx.

Item Details

Item Type:Refereed Article
Keywords:Plasma; Glutathione peroxidase; ELISA; Selenium; Selenoprotein
Research Division:Biomedical and Clinical Sciences
Research Group:Pharmacology and pharmaceutical sciences
Research Field:Pharmaceutical sciences
Objective Division:Health
Objective Group:Other health
Objective Field:Other health not elsewhere classified
UTAS Author:Jacobson, GA (Associate Professor Glenn Jacobson)
UTAS Author:Narkowicz, CK (Dr Christian Narkowicz)
UTAS Author:Tong, YC (Miss Emily Tong)
UTAS Author:Peterson, GM (Professor Gregory Peterson)
ID Code:40237
Year Published:2006
Web of Science® Times Cited:21
Deposited By:Pharmacy
Deposited On:2006-08-01
Last Modified:2011-05-09
Downloads:2 View Download Statistics

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