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Plasma glutathione peroxidase by ELISA and relationship to selenium level
Citation
Jacobson, GA and Narkowicz, CK and Tong, YC and Peterson, GM, Plasma glutathione peroxidase by ELISA and relationship to selenium level, Clinica Chimica Acta, 369, (1) pp. 100-103. ISSN 0009-8981 (2006) [Refereed Article]
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DOI: doi:10.1016/j.cca.2006.01.007
Abstract
Background
Many assays have investigated GPx activity but there are few reports of immunoreactive methods of GPx plasma determinations and their relationship with plasma selenium.
Methods
A cross-sectional analysis of selenium and GPx was carried out on 170 adults randomly selected from the electoral role. Blood samples were collected and analysed for plasma GPx using a commercial enzyme-linked immunosorbent assay (ELISA) kit and plasma selenium using magnetic sector ICP–MS. Further investigations of GPx level were carried out using one-dimensional (1D) and two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE).
Results
Plasma GPx was associated with plasma Se level (r2 = 0.24, P < 0.0001). Mean (range) Se was 108.9 (67.4–268.0) ìg/l. Mean (range) plasma GPx was 18.5 (3.8–43.8) ìg/ml, significantly higher than the theoretical mean GPx of around 6.5–6.9 ìg/ml based on ICP–MS results and the proportion of Se in intact GPx. GPx was higher in males than females and lower in current than non-smokers and ex-smokers. PAGE analysis of glycine dissociated ELISA wells revealed GPx as well as a number of unknown proteins with significant variability between high and low GPx samples.
Conclusions
Plasma GPx is correlated with Se status, however, there is considerable GPx variability between patients and ELISA methods appear to significantly overestimate true plasma GPx.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Plasma; Glutathione peroxidase; ELISA; Selenium; Selenoprotein |
| Research Division: | Medical and Health Sciences |
| Research Group: | Pharmacology and Pharmaceutical Sciences |
| Research Field: | Pharmaceutical Sciences |
| Objective Division: | Health |
| Objective Group: | Other Health |
| Objective Field: | Health not elsewhere classified |
| Author: | Jacobson, GA (Dr Glenn Jacobson) |
| Author: | Narkowicz, CK (Dr Christian Narkowicz) |
| Author: | Tong, YC (Miss Emily Tong) |
| Author: | Peterson, GM (Professor Gregory Peterson) |
| ID Code: | 40237 |
| Year Published: | 2006 |
| Web of Science® Times Cited: | 18 |
| Deposited By: | Pharmacy |
| Deposited On: | 2006-08-01 |
| Last Modified: | 2011-05-09 |
| Downloads: | 2 View Download Statistics |
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