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Potassium activities in cell compartments of salt-grown barley leave

Citation

Cuin, TA and Miller, AJ and Laurie, SA and Leigh, RA, Potassium activities in cell compartments of salt-grown barley leave, Journal of Experimental Botany, 54, (383) pp. 657-661. ISSN 0022-0957 (2003) [Refereed Article]

DOI: doi:10.1093/jxb/erg072

Abstract

Triple-barrelled microelectrodes measuring K+ activity (aK), pH and membrane potential were used to make quantitative measurements of vacuolar and cytosolic aK in epidermal and mesophyll cells of barley plants grown in nutrient solution with 0 or 200 mM added NaCl. Measurements of aK were assigned to the cytosol or vacuole based on the pH measured. In epidermal cells, the salt treatment decreased aK in the vacuole from 224 to 47 mM and in the cytosol from 68 to 15 mM. In contrast, the equivalent changes in the mesophyll were from 235 to 150 mM (vacuole) and 79 to 64 mM (cytosol). Thus mechanisms exist to ameliorate the effects of salt on aK in compartments of mesophyll cells, presumably to minimize any deleterious consequences for photosynthesis. Thermodynamic calculations showed that K+ is actively transported into the vacuole of both epidermal and mesophyll cells of salinized and non-salinized plants. Comparison of the values of aK in K+-replete, non-salinized leaf cells with those previously measured in root cells of plants grown under comparable conditions indicates that cytosolic aK is similar in cells of both organs, but vacuolar aK in leaf cells is approximately twice that in roots. This suggests differences in the regulation of vacuolar aK, but not cytosolic aK, in leaf and root cells.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Biochemistry and Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
Author:Cuin, TA (Dr Tracey Cuin)
ID Code:39813
Year Published:2003
Web of Science® Times Cited:136
Deposited By:Agricultural Science
Deposited On:2006-07-04
Last Modified:2006-07-04
Downloads:0

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