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Use of optical density as a measure of Claviceps africana conidial suspension concentration
journal contribution
posted on 2023-05-16, 17:55 authored by Herde, DJ, Ryley, MJ, Scott FosterScott Foster, Galea, VJ, Henzell, RG, Jordan, DRSorghum ergot, caused by Claviceps africana, has remained a major disease problem in Australia since it was first recorded in 1996, and is the focus of a range of biological and integrated management research. Artificial inoculation using conidial suspensions is an important tool in this research. Ergot infection is greatly influenced by environmental factors, so it is important to reduce controllable sources of variation such as inoculum concentration. The use of optical density was tested as a method of quantifying conidial suspensions of C. africana, as an alternative to haemocytometer counts. This method was found to be accurate and time efficient, with possible applications in other disease systems. © Australasian Plant Pathology Society 2006.
History
Publication title
Australasian Plant PathologyVolume
35Pagination
77-80ISSN
0815-3191Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
CSIROPlace of publication
AustraliaRepository Status
- Restricted