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A multiplex PCR for β-lactamase genes of Haemophilus influenzae and description of a new blaTEM promoter variant

Citation

Tristram, SG and Nichols, S, A multiplex PCR for β-lactamase genes of Haemophilus influenzae and description of a new blaTEM promoter variant, Journal of Antimicrobial Chemotherapy, 58, (1) pp. 183-185. ISSN 0305-7453 (2006) [Refereed Article]

DOI: doi:10.1093/jac/dkl150

Abstract

Objectives: To establish a multiplex PCR to detect and differentiate the β-lactamase genes of Haemophilus influenzae. Methods: A collection of H. influenzae isolates with a range of different β-lactamase genes were tested in parallel with individual PCRs and the multiplex assay developed in this study. Results: The multiplex method was found to be superior to previous methods and was able to correctly detect and differentiate bla ROB-1 and the variants of blaTEM present in the strains tested, including a previously unrecognized variant associated with a 54 bp insertion in the promoter region. Conclusions: The multiplex PCR will be a useful tool for the surveillance of β-lactamase genes in H. influenzae. © 2006 Oxford University Press.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Medical Microbiology
Research Field:Medical Bacteriology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:Tristram, SG (Dr Stephen Tristram)
Author:Nichols, S (Mr Scott Nichols)
ID Code:39395
Year Published:2006
Web of Science® Times Cited:13
Deposited By:Health Sciences A
Deposited On:2006-08-01
Last Modified:2007-04-04
Downloads:0

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