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A multiplex PCR for β-lactamase genes of Haemophilus influenzae and description of a new blaTEM promoter variant
Citation
Tristram, SG and Nichols, S, A multiplex PCR for β-lactamase genes of Haemophilus influenzae and description of a new blaTEM promoter variant, Journal of Antimicrobial Chemotherapy, 58, (1) pp. 183-185. ISSN 0305-7453 (2006) [Refereed Article]
Abstract
Objectives: To establish a multiplex PCR to detect and differentiate the β-lactamase genes of Haemophilus influenzae. Methods: A collection of H. influenzae isolates with a range of different β-lactamase genes were tested in parallel with individual PCRs and the multiplex assay developed in this study. Results: The multiplex method was found to be superior to previous methods and was able to correctly detect and differentiate bla ROB-1 and the variants of blaTEM present in the strains tested, including a previously unrecognized variant associated with a 54 bp insertion in the promoter region. Conclusions: The multiplex PCR will be a useful tool for the surveillance of β-lactamase genes in H. influenzae. © 2006 Oxford University Press.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Medical and Health Sciences |
| Research Group: | Medical Microbiology |
| Research Field: | Medical Bacteriology |
| Objective Division: | Health |
| Objective Group: | Clinical Health (Organs, Diseases and Abnormal Conditions) |
| Objective Field: | Infectious Diseases |
| Author: | Tristram, SG (Dr Stephen Tristram) |
| Author: | Nichols, S (Mr Scott Nichols) |
| ID Code: | 39395 |
| Year Published: | 2006 |
| Web of Science® Times Cited: | 13 |
| Deposited By: | Health Sciences A |
| Deposited On: | 2006-08-01 |
| Last Modified: | 2007-04-04 |
| Downloads: | 0 |
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