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A multiplex PCR for β-lactamase genes of Haemophilus influenzae and description of a new blaTEM promoter variant
Citation
Tristram, SG and Nichols, S, A multiplex PCR for β-lactamase genes of Haemophilus influenzae and description of a new blaTEM promoter variant, Journal of Antimicrobial Chemotherapy, 58, (1) pp. 183-185. ISSN 0305-7453 (2006) [Refereed Article]
Abstract
Objectives: To establish a multiplex PCR to detect and differentiate the β-lactamase genes of Haemophilus influenzae. Methods: A collection of H. influenzae isolates with a range of different β-lactamase genes were tested in parallel with individual PCRs and the multiplex assay developed in this study. Results: The multiplex method was found to be superior to previous methods and was able to correctly detect and differentiate bla ROB-1 and the variants of blaTEM present in the strains tested, including a previously unrecognized variant associated with a 54 bp insertion in the promoter region. Conclusions: The multiplex PCR will be a useful tool for the surveillance of β-lactamase genes in H. influenzae. © 2006 Oxford University Press.
Item Details
Item Type: | Refereed Article |
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Research Division: | Biomedical and Clinical Sciences |
Research Group: | Medical microbiology |
Research Field: | Medical bacteriology |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Tristram, SG (Dr Stephen Tristram) |
UTAS Author: | Nichols, S (Mr Scott Nichols) |
ID Code: | 39395 |
Year Published: | 2006 |
Web of Science® Times Cited: | 18 |
Deposited By: | Health Sciences A |
Deposited On: | 2006-08-01 |
Last Modified: | 2007-04-04 |
Downloads: | 0 |
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