eCite Digital Repository

Expression of transient receptor potential vanilloid 1 (TRPV1) and 2 (TRPV2) in human peripheral blood

Citation

Saunders, CIM and Kunde, DA and Crawford, A and Geraghty, DP, Expression of transient receptor potential vanilloid 1 (TRPV1) and 2 (TRPV2) in human peripheral blood, Molecular Immunology, 44, (6) pp. 1429-35. . ISSN 0161-5890 (2007) [Refereed Article]


Preview
PDF
Restricted - Request a copy
438Kb
  

Copyright Statement

The definitive version is available at http://www.sciencedirect.com

Official URL: http://www.sciencedirect.com

DOI: doi:10.1016/j.molimm.2006.04.027

Abstract

The vanilloid receptor family of cation channels includes the capsaicin-sensitive, proton- and heat-activated TRPV1 and noxious heat-activated TRPV2. The present study demonstrates both gene and protein expression of TRPV1 and TRPV2 in human peripheral blood cells (PBCs) using molecular and immunocytochemical techniques. Using reverse-transcription polymerase chain reaction (RT-PCR) and quantitative real-time RTPCR (qRT-PCR), TRPV1 and TRPV2mRNAwas detected inmRNAisolated from human whole peripheral blood. Using qRT-PCR, TRPV2mRNA was highly expressed in human whole blood isolates (9.33㊣1.19℅104 copies per 106 copies of the housekeeping gene GAPDH), whereas TRPV1 message was detected at >150-fold lower levels (638㊣121 copies per 106 copies GAPDH). At the protein level, TRPV1 and TRPV2 activity was determined immunocytochemically in a lymphocyte-enriched mononuclear cell preparation (83㊣2% lymphocytes). Cells were labelled with rabbit anti-TRPV1 or goat anti-TRPV2 (1:500) and subsequently labelled with goat Texas red- (TRPV1) or FITC-(TRPV2) conjugated secondary antibodies (1:1000). All cells demonstrated punctate TRPV1-immunoreactivity, which appeared to be on the plasma membrane and in the cytoplasm. In contrast, cells within subjects appeared to express the TRPV1 protein at varying intensities. TRPV2-immunoreactivity appeared diffuse. This is the first study to demonstrate the presence of both TRPV1 and TRPV2 in human peripheral lymphocytes. Further studies need to be undertaken in order to determine the role of TRPV channels in these cells.

Item Details

Item Type:Refereed Article
Keywords:Transient receptor potential V1; Transient receptor potential V2; Human mononuclear cells; Immunocytochemistry; Real-time PCR
Research Division:Medical and Health Sciences
Research Group:Medical Biochemistry and Metabolomics
Research Field:Medical Biochemistry: Nucleic Acids
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Blood Disorders
Author:Saunders, CIM (Dr Cassie Saunders)
Author:Kunde, DA (Dr Dale Kunde)
Author:Crawford, A (Miss Amanda Crawford)
Author:Geraghty, DP (Professor Dominic Geraghty)
ID Code:39383
Year Published:2007
Web of Science® Times Cited:47
Deposited By:Health Sciences A
Deposited On:2007-08-01
Last Modified:2009-10-09
Downloads:4 View Download Statistics

Repository Staff Only: item control page