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Expression of transient receptor potential vanilloid 1 (TRPV1) and 2 (TRPV2) in human peripheral blood


Saunders, CIM and Kunde, DA and Crawford, A and Geraghty, DP, Expression of transient receptor potential vanilloid 1 (TRPV1) and 2 (TRPV2) in human peripheral blood, Molecular Immunology, 44, (6) pp. 1429-35. ISSN 0161-5890 (2007) [Refereed Article]

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DOI: doi:10.1016/j.molimm.2006.04.027


The vanilloid receptor family of cation channels includes the capsaicin-sensitive, proton- and heat-activated TRPV1 and noxious heat-activated TRPV2. The present study demonstrates both gene and protein expression of TRPV1 and TRPV2 in human peripheral blood cells (PBCs) using molecular and immunocytochemical techniques. Using reverse-transcription polymerase chain reaction (RT-PCR) and quantitative real-time RTPCR (qRT-PCR), TRPV1 and TRPV2mRNAwas detected inmRNAisolated from human whole peripheral blood. Using qRT-PCR, TRPV2mRNA was highly expressed in human whole blood isolates (9.33㊣1.19℅104 copies per 106 copies of the housekeeping gene GAPDH), whereas TRPV1 message was detected at >150-fold lower levels (638㊣121 copies per 106 copies GAPDH). At the protein level, TRPV1 and TRPV2 activity was determined immunocytochemically in a lymphocyte-enriched mononuclear cell preparation (83㊣2% lymphocytes). Cells were labelled with rabbit anti-TRPV1 or goat anti-TRPV2 (1:500) and subsequently labelled with goat Texas red- (TRPV1) or FITC-(TRPV2) conjugated secondary antibodies (1:1000). All cells demonstrated punctate TRPV1-immunoreactivity, which appeared to be on the plasma membrane and in the cytoplasm. In contrast, cells within subjects appeared to express the TRPV1 protein at varying intensities. TRPV2-immunoreactivity appeared diffuse. This is the first study to demonstrate the presence of both TRPV1 and TRPV2 in human peripheral lymphocytes. Further studies need to be undertaken in order to determine the role of TRPV channels in these cells.

Item Details

Item Type:Refereed Article
Keywords:Transient receptor potential V1; Transient receptor potential V2; Human mononuclear cells; Immunocytochemistry; Real-time PCR
Research Division:Biomedical and Clinical Sciences
Research Group:Medical biochemistry and metabolomics
Research Field:Medical biochemistry - nucleic acids
Objective Division:Health
Objective Group:Clinical health
Objective Field:Diagnosis of human diseases and conditions
UTAS Author:Saunders, CIM (Dr Cassie Saunders)
UTAS Author:Kunde, DA (Dr Dale Kunde)
UTAS Author:Crawford, A (Miss Amanda Crawford)
UTAS Author:Geraghty, DP (Professor Dominic Geraghty)
ID Code:39383
Year Published:2007
Web of Science® Times Cited:79
Deposited By:Health Sciences A
Deposited On:2007-08-01
Last Modified:2016-06-20
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