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Genetic Screening for Prey in the Gut Contents from a Giant Squid (Architeuthis sp.)

Citation

Deagle, BE and Jarman, SN and Pemberton, D and Gales, NJ, Genetic Screening for Prey in the Gut Contents from a Giant Squid (Architeuthis sp.), Journal of Heredity, 96, (4) pp. 417-423. ISSN 0022-1503 (2005) [Refereed Article]

DOI: doi:10.1093/jhered/esi036

Abstract

Giant squids (Architeuthis sp.) remain mysterious; they have evaded observation and are rarely taken from their deep sea habitat. Information on the diet of Architeuthis is scarce due to the limited number of specimens with morphologically recognizable remains in their digestive tracts. We explored the use of polymerase chain reaction (PCR)-based methods for detection of DNA in the prey remains and amorphous slurry from an Architeuthis gut sample. The DNA region amplified varied in size, allowing separation of fish and squid components. Sequence comparisons identified fish prey as Macruronus novaezelandiae. Isolation of Architeuthis DNA from an ingested tentacle and the presence of chitin fragments indicate cannibalism occurs in giant squid. Denaturing gradient gel electrophoresis was used to screen for less common DNA types, revealing a high frequency of PCR-generated false alleles, but no additional prey species. © The American Genetic Association. 2005. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Environmental Sciences
Research Group:Environmental Science and Management
Research Field:Wildlife and Habitat Management
Objective Division:Environment
Objective Group:Ecosystem Assessment and Management
Objective Field:Ecosystem Assessment and Management of Antarctic and Sub-Antarctic Environments
Author:Deagle, BE (Dr Bruce Deagle)
ID Code:38896
Year Published:2005
Web of Science® Times Cited:44
Deposited By:TAFI - Zoology
Deposited On:2005-08-01
Last Modified:2006-05-02
Downloads:0

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