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Functional and metabolic evidence for two different vanilloid (VN1 and VN2) receptors in perfused rat hindlimb

Citation

Colquhoun, EQ and Eldershaw, TPD and Bennett, KL and Hall, JL and Dora, KA and Clark, MG, Functional and metabolic evidence for two different vanilloid (VN1 and VN2) receptors in perfused rat hindlimb, Life Sciences, 57, (2) pp. 91-102. ISSN 0024-3205 (1995) [Refereed Article]

DOI: doi:10.1016/0024-3205(95)00250-A

Abstract

Vanilloid spice principles, including capsaicin, stimulate vasoconstriction in the rat hindlimb perfused at constant flow and, depending on dose, either stimulate or inhibit oxygen consumption by this vascular bed. We now present metabolic and functional evidence for two different vanilloid (VN 1 and VN 2) receptor types. These receptors can be distinguished on the basis of their differing agonist affinity for capsacin, their different calcium and oxygen dependencies for inducing vasoconstriction, and whether they stimulate, or inhibit, oxygen consumption. The higher affinity vanilloid receptor, VN 1 can be distinguished on the basis of initiating vasoconstriction at low doses of capsaicin and stimultaneously stimulating oxygen consumption. Its apparent biological function is dependent on the presence of oxygen and external calcium. In contrast, the lower affinity receptor, VN 2 induces vasoconstriction associated with inhibition of oxygen consumption. Its vasoconstriction action can occur independently of either external calcium ions, or the presence of oxygen in the perfusate.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Pharmacology and Pharmaceutical Sciences
Research Field:Basic Pharmacology
Objective Division:Health
Objective Group:Other Health
Objective Field:Health not elsewhere classified
Author:Colquhoun, EQ (Dr Eric Colquhoun)
Author:Eldershaw, TPD (Mr Tristram Eldershaw)
Author:Hall, JL (Ms Jenny Hall)
Author:Dora, KA (Dr Kim Dora)
Author:Clark, MG (Professor Michael Clark)
ID Code:3883
Year Published:1995
Web of Science® Times Cited:21
Deposited By:Biochemistry
Deposited On:1995-08-01
Last Modified:2011-11-18
Downloads:0

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