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Peculiarities of host cholesterol transport to the unique intracellular vacuole containing Toxoplasma

journal contribution
posted on 2023-05-16, 17:33 authored by Sehgal, A, Silvana BettiolSilvana Bettiol, Pypaert, M, Wenk, MR, Kaasch, A, Blader, IJ, Joiner, KA, Coppens, I
The intracellular protozoan Toxoplasma gondii is auxo-trophic for low-density lipoprotein (LDL)-derived cholesterol (C). We previously showed that T. gondii scavenges this essential lipid from host endolysosomal compartments and that C delivery to the parasitophorous vacuole (PV) does not require transit through host Golgi or encdoplasmic reticulum. In this study, we explore the itinerary of C from the host endolysosomes; to the PV. Labeled C incorporated into LDL is rapidly detected in intravacuolar parasites and partially esterified by the parasites. In contrast to diverse mammalian organelles, the post-endolysosomal transfer of to the PV does not involve the host plasma membrane as an intermediate. Nevertheless, the PV membrane is accessible to extracellular sterol acceptors, suggesting C trafficking from intracellular parasites to host plasma membrane. C movement to the PV requires temperatures permissive for vesicular transport, metabolic energy and functional microtubules Host caveolae vesicles and the sterol carrier protein-2 do not participate in this process. Proteolytic treatment of purified PV or free parasites abolishes C acquisition by the parasites. Altogether, these results support a vesicular transport system from host endolysosomes to the PV, and a requirement for PV membrane and parasite plasma membrane proteins in C delivery to T. gondii. Copyright © Blackwell Munksgaard 2005.

History

Publication title

Traffic

Volume

6

Issue

12

Pagination

1125-1141

ISSN

1398-9219

Department/School

Tasmanian School of Medicine

Publisher

Blackwell Publishing

Place of publication

Oxford, England

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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