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Contactless conductivity detection of synthetic polymers in non-aqueous size-exclusion electrokinetic chromatography


Oudhoff, KA and Macka, M and Haddad, PR and Schoenmakers, PJ and Kok, WT, Contactless conductivity detection of synthetic polymers in non-aqueous size-exclusion electrokinetic chromatography, Journal of Chromatography A, 1068, (1) pp. 183-187. ISSN 0021-9673 (2005) [Refereed Article]

DOI: doi:10.1016/j.chroma.2004.11.081


A capacitively coupled contactless conductivity detection (CCD) system has been applied for the detection of neutral synthetic polymers in capillary size-exclusion electrokinetic chromatography (SEEC). Polystyrene standards, that were used as a model compounds, were separated on a capillary column packed with porous 10 μm silica particles with an electrokinetically driven mobile phase, and detected by CCD and UV detection simultaneously. Mass-calibration curves for polystyrene were constructed. Satisfactory results were obtained for the linearity, the run-to-run repeatability (<0.2% for the relative retention and <4% for the peak area) and the robustness of the detector. One of the major issues in this preliminary study was to investigate the origin of the peaks observed for the polystyrene standards. The effect of the molar mass of the polystyrenes on the sensitivity was small. Therefore, the signals obtained could not be explained as the result of an increased viscosity and a decreased solution conductivity of the solute zone. An alternative hypothesis is suggested, and recommendations for further research are given. © 2004 Elsevier B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Oudhoff, KA (Dr Kathalijne Oudhoff)
UTAS Author:Macka, M (Professor Mirek Macka)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:38076
Year Published:2005
Web of Science® Times Cited:14
Deposited By:Chemistry
Deposited On:2005-08-01
Last Modified:2009-11-18

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