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Latex-Coated Polymeric Monolithic Ion-Exchange Stationary Phases. 2. Micro-ion Chromatography


Zakaria, P and Hutchinson, J and Avdalovic, N and Liu, Y and Haddad, PR, Latex-Coated Polymeric Monolithic Ion-Exchange Stationary Phases. 2. Micro-ion Chromatography, Analytical Chemistry, 77, (2) pp. 417-423. ISSN 0003-2700 (2005) [Refereed Article]


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Copyright 2005 American Chemical Society - Reproduced by permission of The Royal Society of Chemistry (RSC)

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DOI: doi:10.1021/ac048747l


Latex-coated monolithic polymeric stationary phases are used for micro-ion chromatography (μ-IC) of inorganic anions. Monolithic columns were prepared by the in situ polymerization of butyl methacrylate, ethylene dimethacrylate, and 2-acrylamido-2-methyl-1-propanesulfonic acid within fused-silica capillaries of varying internal diameters. Introduction of ion-exchange sites was achieved by coating the anionic polymeric monolith with either Dionex AS10 or Dionex AS18 quaternary ammonium functionalized latex particles to give total ion-exchange capacities in the range 9−24 nequiv for a 30-cm column. The resultant μ-IC columns were used for the separation of anionic analytes using chloride or acetate as the eluent-competing ion and direct UV spectrophotometric detection at 195 nm or using hydroxide as the eluent-competing ion and suppressed or nonsuppressed contactless conductivity detection. Separation efficiencies of 13 000 plates/m were observed (for iodate), and separation efficiency was maintained for large increases in flow rate (up to 42 μL/min, corresponding to a linear flow velocity of 18.5 mm/s), enabling highly reproducible, rapid separations to be achieved (seven analyte anions in less than 2 min). Use of a hollow fiber micromembrane suppressor enabled effective suppression of hydroxide eluents over the range 0.5−5.0 mM, thereby permitting suppressed conductivity detection to be performed. However, the relatively large size of the suppressor resulted in reduced separation efficiencies (e.g., 5400 plates/m for iodate). Detection limits obtained with suppressed conductivity detection were in the range 0.4−1.2 μM.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Zakaria, P (Dr Philip Zakaria)
UTAS Author:Hutchinson, J (Dr Joseph Hutchinson)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:38000
Year Published:2005
Web of Science® Times Cited:104
Deposited By:Chemistry
Deposited On:2005-08-01
Last Modified:2010-02-01
Downloads:526 View Download Statistics

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