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Isoelectric Buffers for Capillary Electrophoresis. 2. Bismorpholine Derivative of a Carboxylic Acid as a Low Molecular Weight Isoelectric Buffer

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posted on 2023-05-16, 17:26 authored by Thomas RodemannThomas Rodemann, Johns, CA, Yang, W, Paul HaddadPaul Haddad, Miroslav MackaMiroslav Macka
A new compound class of synthetic isoelectric buffers is introduced, designed as a small molecule with one fully or prevailingly dissociated acidic group (such as sulfonic or carboxylic) and two partly pronated (buffering) basic amino groups attached onto a hydrophilic UV-transparent backbone. As an example, a new isoelectric compound 2,2-bis(4-morpholinylmethyl)propanoic acid (BMMPA) was synthesized by attaching two morpholine groups onto a molecule of pivalic acid. It was characterized as having an isoelectric point pI = 6.5 and exhibiting satisfactory buffering capacity at the pI. Solutions of BMMPA are transparent down to the low-UV spectral region, thus making it a potentially suitable buffer for a number of separation methods. Its use in capillary electrophoresis was demonstrated in a separation system for indirect photometric detection of anions based on an electrolyte with the anionic dye Orange G as the indirect detection probe and using BMMPA as a buffer. The use of an isoelectric buffering compound brings the advantages of a buffered electrolyte without the concomitant introduction of co-ions that would be detrimental to the indirect detection process. Submicromole per liter limits of detection for a number of inorganic and small organic ions were achieved. Optimal structural properties of the isoelectric buffer with respect to its buffering properties are discussed.

History

Publication title

Analytical Chemistry

Volume

77

Pagination

120-125

ISSN

0003-2700

Department/School

School of Natural Sciences

Publisher

American Chemical Society

Place of publication

Washington

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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