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Acute glucosamine-induced insulin resistance in muscle in vivo is associated with impaired capillary recruitment
Citation
Wallis, MG and Smith, ME and Kolka, CM and Zhang, L and Richards, SM and Rattigan, S and Clark, MG, Acute glucosamine-induced insulin resistance in muscle in vivo is associated with impaired capillary recruitment, Diabetologia, 48, (10) pp. 2131-2139. ISSN 0012-186X (2005) [Refereed Article]
DOI: doi:10.1007/s00125-005-1887-z
Abstract
Aims/hypothesis: Glucose toxicity and glucosamine-induced insulin resistance have been attributed to products of glucosamine metabolism. In addition, endothelial cell nitric oxide synthase is inhibited by glucosamine. Since insulin has endothelial nitric-oxide-dependent vasodilatory effects in muscle, we hypothesise that glucosamine-induced insulin resistance in muscle in vivo is associated with impaired vascular responses including capillary recruitment. Materials and methods: Glucosamine (6.48 mg kg-1 min-1 for 3 h) was infused with or without insulin (10 mU kg -1 min-1) into anaesthetised rats under euglycaemic conditions. Results: Glucosamine infusion alone increased blood glucosamine (1.9±0.1 mmol/l) and glucose (5.4±0.2 to 7.7±0.3 mmol/l) (p<0.05) but not insulin. Glucosamine induced both hepatic and muscle insulin resistance as evident from measures of glucose appearance and disposal as well as hind-leg glucose uptake, which was inhibited by approx. 50% (p<0.05). Insulin-mediated increases in femoral arterial blood flow and capillary recruitment were completely blocked by glucosamine. Conclusion/interpretation: Glucosamine mediates a major impairment of insulin action in muscle vasculature associated with the insulin resistance of muscle. Further studies will be required to assess whether the impaired capillary recruitment contributes to insulin resistance. © Springer-Verlag 2005.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Medical and Health Sciences |
| Research Group: | Clinical Sciences |
| Research Field: | Endocrinology |
| Objective Division: | Health |
| Objective Group: | Clinical Health (Organs, Diseases and Abnormal Conditions) |
| Objective Field: | Diabetes |
| Author: | Smith, ME (Mrs Maree Smith) |
| Author: | Kolka, CM (Miss Cathryn Kolka) |
| Author: | Zhang, L (Mr Lejun Zhang) |
| Author: | Richards, SM (Dr Stephen Richards) |
| Author: | Rattigan, S (Professor Stephen Rattigan) |
| Author: | Clark, MG (Professor Michael Clark) |
| ID Code: | 37451 |
| Year Published: | 2005 |
| Funding Support: | National Health and Medical Research Council (352600) |
| Web of Science® Times Cited: | 20 |
| Deposited By: | Biochemistry |
| Deposited On: | 2005-08-01 |
| Last Modified: | 2011-10-03 |
| Downloads: | 0 |
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