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Microvascular flow routes in muscle controlled by vasoconstrictors

Citation

Zhang, L and Newman, JMB and Richards, SM and Rattigan, S and Clark, MG, Microvascular flow routes in muscle controlled by vasoconstrictors, Microvascular Research, 70, (1) pp. 7-16. ISSN 0026-2862 (2005) [Refereed Article]

DOI: doi:10.1016/j.mvr.2005.06.001

Abstract

Vasoconstrictors can either increase or decrease metabolism of the constant flow pump-perfused rat hindlimb. In addition, there is indirect evidence from vascular casts, surface fluorometry, dye entrapment studies, and fluorescent microsphere mapping of flow that this may be due to redistribution of flow between putatively nutritive and non-nutritive routes within muscle. In the present study, we used two methods in an attempt to identify perfused nutritive and non-nutritive vessels in muscle sections: (i) a combination of perfusion fixation with glutaraldehyde and post-perfusion Griffonia simplicifolia lectin and (ii) perfusion with rhodamine-dextran70 (lysine fixable) and post-fixation with formaldehyde. Perfusions involved vehicle only (control, a mix of nutritive and non-nutritive flow), 15 nM angiotensin II (AII) to increase, or 1 μM serotonin (5-HT) to decrease nutritive flow. Microscopic examination of muscle sections following AII showed an increase in perfused capillaries with fewer areas of under-perfusion, relative to control. In contrast, 5-HT caused a marked decrease in perfused capillaries relative to control and evidence that flow was carried by connective tissue vessels that on average were of greater diameter and were more sparsely distributed than capillaries. It is concluded that vasoconstrictors that alter hindlimb metabolism do so by intra-muscle redistribution between capillaries (nutritive) and non-nutritive (connective tissue) vessels within each muscle. © 2005 Elsevier Inc. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Endocrinology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cardiovascular System and Diseases
Author:Zhang, L (Mr Lejun Zhang)
Author:Newman, JMB (Dr John Newman)
Author:Richards, SM (Dr Stephen Richards)
Author:Rattigan, S (Professor Stephen Rattigan)
Author:Clark, MG (Professor Michael Clark)
ID Code:37450
Year Published:2005
Funding Support:National Health and Medical Research Council (253900)
Web of Science® Times Cited:11
Deposited By:Biochemistry
Deposited On:2005-08-01
Last Modified:2006-03-14
Downloads:0

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