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Endothelial Na+ -D-glucose cotransporter: No Role in Insulin-mediated Glucose Uptake

Citation

Kolka, CM and Rattigan, S and Richards, SM and Barrett EJ, EJ and Clark, MG, Endothelial Na+ -D-glucose cotransporter: No Role in Insulin-mediated Glucose Uptake , Hormone and Metabolic Research, 37, (11) pp. 657-661. ISSN 0018-5043 (2005) [Refereed Article]

DOI: doi:10.1055/s-2005-870574

Abstract

A recent report indicates that the Na+-D-glucose cotransporter SGLT1 is present in capillaries of skeletal muscle and is required for insulin-mediated glucose uptake in myocytes. This result is based on the complete inhibition of insulin-mediated muscle glucose uptake by phlorizin, an inhibitor of SGLT1. Using the pump-perfused rat hind limb, we measured glucose uptake, lactate efflux, and radioactive 2-deoxyglucose uptake into individual muscles with saline (control), phlorizin, insulin, and insulin plus phlorizin, as well as with saline and insulin using normal and low Na+ perfusion buffer. Insulin-mediated glucose uptake was not inhibited after correction for phlorizin interference in the glucose assay. Lactate efflux and 2-deoxyglucose uptake by individual muscles were unaffected by phlorizin. Low Na+ buffer did not affect insulin-mediated glucose uptake, lactate efflux, or 2-deoxyglucose uptake. We conclude that endothelial SGLT1 exerts no barrier for glucose delivery to myocytes. © Georg Thieme Verlag KG Stuttgart.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Endocrinology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Diabetes
Author:Kolka, CM (Miss Cathryn Kolka)
Author:Rattigan, S (Professor Stephen Rattigan)
Author:Richards, SM (Dr Stephen Richards)
Author:Clark, MG (Professor Michael Clark)
ID Code:37449
Year Published:2005
Web of Science® Times Cited:2
Deposited By:Biochemistry
Deposited On:2005-08-01
Last Modified:2011-10-07
Downloads:0

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