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Different properties of SV channels in root vacuoles from near isogenic A1-tolerant and A1-sensitive wheat cultivars

Citation

Wherrett, TC and Shabala, SN and Pottosin, I, Different properties of SV channels in root vacuoles from near isogenic A1-tolerant and A1-sensitive wheat cultivars, FEBS Letters, 579, (30) pp. 6890-6894. ISSN 0014-5793 (2005) [Refereed Article]

DOI: doi:10.1016/j.febslet.2005.11.038

Abstract

Patch-clamp experiments revealed that near isogenic ET8 (Al-tolerant) and ES8 (Al-sensitive) wheat cultivars differed significantly in slow vacuolar channel properties. Under control conditions, whole vacuole currents displayed faster deactivation in ES8. Application of 1.4 μM vacuolar Al3+ caused a 20 mV increase in the activation threshold and slowed activation kinetics in ET8 but not in ES8. Channel density was about 30% higher in ES8 than ET8, and was not altered by 24 h aluminium pre-treatment. However, the activation threshold was reduced in Al-pre-treated ES8. Overall, our data suggests that Alt1 locus may control more than the plasma membrane malate channel in wheat. © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Biochemistry and Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Wheat
Author:Wherrett, TC (Mr Timothy Wherrett)
Author:Shabala, SN (Professor Sergey Shabala)
ID Code:36555
Year Published:2005
Web of Science® Times Cited:8
Deposited By:Agricultural Science
Deposited On:2005-08-01
Last Modified:2006-05-04
Downloads:0

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