eCite Digital Repository

Comprehensive two-dimensional gas chromatography (GC x GC) and GC x GC-quadrupole MS analysis of Asian and American ginseng

Citation

Shellie, RA and Marriott, P and Huie, CW, Comprehensive two-dimensional gas chromatography (GC x GC) and GC x GC-quadrupole MS analysis of Asian and American ginseng, Journal of Separation Science, 26, (12/13) pp. 1185-1192. ISSN 1615-9306 (2003) [Refereed Article]


Preview
PDF
Restricted - Request a copy
637Kb
  

Copyright Statement

The definitive published version is available online at: http://interscience.wiley.com

DOI: doi:10.1002/jssc.200301404

Abstract

Extracts of the roots of Panax (ginseng) species are analysed using comprehensive two-dimensional gas chromatography. Comparison of comprehensive two-dimensional chromatograms reveals the presence of numerous common components, and possible species-specific components are also observed. Two-dimensional bubble plot presentations of GC×GC(FID) data allow ready qualitative and semi-quantitative comparison of the extracts. The use of GC×GC-quadrupole MS (GC×GC-qMS), operated at a scanning rate of 20 Hz, in conjunction with retention data, permits identification of separated (major) components. Whilst this scan speed will be insufficient for reliable quantitative analysis, considerably improved quality of uncorrected spectra, arising from the enhanced separation over single column GC-MS analysis is apparent. Spectral match qualities of up to 99% are found for a number of components. Comparison of GC×GC-qMS with GC×GC(FID) indicates that good correspondence of peak position patterns is achieved, which aids component identification. It is shown that (quadrupole) mass spectrometric detection is a useful tool for provision of identification of separated compounds in high-resolution comprehensive two-dimensional gas chromatography.

Item Details

Item Type:Refereed Article
Keywords:Comprehensive gas chromatography • Mass spectrometry • Panax ginseng • Panax notoginseng • Panax quinquefolium • GC×GC-MS
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Manufacturing
Objective Group:Instrumentation
Objective Field:Scientific Instruments
Author:Shellie, RA (Associate Professor Robert Shellie)
ID Code:36248
Year Published:2003
Web of Science® Times Cited:73
Deposited By:Chemistry
Deposited On:2005-11-15
Last Modified:2010-04-09
Downloads:3 View Download Statistics

Repository Staff Only: item control page