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Targeted Multidimensional Gas Chromatography Using Microswitching and Cryogenic Modulation

Citation

Marriott, P and Dunn, M and Shellie, RA and Morrison, P, Targeted Multidimensional Gas Chromatography Using Microswitching and Cryogenic Modulation, Analytical Chemistry, 75, (20) pp. 5532-5538. ISSN 0003-2700 (2003) [Refereed Article]


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Copyright Statement

© 2003 American Chemical Society

DOI: doi:10.1021/ac034492k

Abstract

A new method is described that allows fast target analysis in multidimensional gas chromatography by using a microswitching valve between two GC columns, with cryogenic trapping and rapid re-injection of trapped solutes in the second dimension. The essence of the procedure is that heart-cut fractions from the first column (1D) can be selectively transferred to column 2 (2D), where a moveable cryogenic trap first focuses the transferred solute(s) at the head of the second column and then permits their facile rapid analysis on 2D. Since 2D is a short narrow-bore column, which exhibits very fast analysis (on the order of a few seconds elution), peak responses (heights) are significantly enhanced (by up to 40-fold). Additionally, by using a 2D phase of a selectivity different from that used for 1D, it is possible to also separate components that are not resolved on the first column and to increase the resolution for other compounds. The heart-cut valve isolates the section(s) of solutes of interest from the first column separation, and this provides a considerable simplification to the chromatogramin addition to the separation and sensitivity advantages. By using this method, multidimensional gas chromatography with multiple heart-cuts can be completed within the same time as the primary column separation. Since the described method permits non-heart-cut fractions to be transferred to a monitor detector, normal detection of these fractions is still permitted. By modulation of the cryotrap, it is also possible to achieve comprehensive two-dimensional gas chromatography for the heart-cut fractions; however, only those compounds passed to the second, separation column, which passes through the cryotrap, will be subjected to GC×GC analysis. The technique and the various modes of operation are described in this paper.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Manufacturing
Objective Group:Instrumentation
Objective Field:Scientific Instruments
Author:Shellie, RA (Associate Professor Robert Shellie)
ID Code:36242
Year Published:2003
Web of Science® Times Cited:34
Deposited By:Chemistry
Deposited On:2005-11-15
Last Modified:2010-03-16
Downloads:0

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