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Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC GC-TOF) for high resolution metabolomics: Biomarker discovery on spleen tissue extracts of obese NZO compared to lean C57BL/6 mice

Citation

Welthagen, W and Shellie, RA and Spranger, J and Ristow, M and Zimmerman, R and Fiehn, O, Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC GC-TOF) for high resolution metabolomics: Biomarker discovery on spleen tissue extracts of obese NZO compared to lean C57BL/6 mice, Metabolomics, 1, (1) pp. 65-73. ISSN 1573-3882 (2005) [Refereed Article]

DOI: doi:10.1007/s11306-005-1108-2

Abstract

Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF) was applied for the analysis of complex metabolite profiles from mouse spleen. The resulting two-dimensional chromatograms proved that mass spectral quality and sensitivity were largely improved by the enhanced resolution and zone compression, which are features of GC × GC operation, when compared to classical one-dimensional GC-TOF methods. The improved peak capacity of GC × GC allowed for peaks to be detected that could previously not be separated in one-dimensional GC. A measure of the combined power of chromatographic and mass spectral deconvolution resolution is called "analytical purity", with higher values indicating less pure peaks. GC × GC-TOF lead to the detection of 1200 compounds with purity better than 0.2, compared to 500 compounds with purity up to 2.5 in one-dimensional GC-TOF. The compounds identified include many of the compounds previously reported in NMR studies and other methods on mammalian tissues. Spleen samples of several obese NZO mice and lean C57BL/6 control strains were analyzed in order to demonstrate the applicability of GC × GC-TOF for biomarker identification. © 2005 Springer Science+Business Media, Inc

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Manufacturing
Objective Group:Instrumentation
Objective Field:Scientific instruments
UTAS Author:Shellie, RA (Associate Professor Robert Shellie)
ID Code:36241
Year Published:2005
Web of Science® Times Cited:129
Deposited By:Chemistry
Deposited On:2005-11-17
Last Modified:2012-04-20
Downloads:0

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