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Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC GC-TOF) for high resolution metabolomics: Biomarker discovery on spleen tissue extracts of obese NZO compared to lean C57BL/6 mice
Citation
Welthagen, W and Shellie, RA and Spranger, J and Ristow, M and Zimmerman, R and Fiehn, O, Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC GC-TOF) for high resolution metabolomics: Biomarker discovery on spleen tissue extracts of obese NZO compared to lean C57BL/6 mice, Metabolomics, 1, (1) pp. 65-73. ISSN 1573-3882 (2005) [Refereed Article]
DOI: doi:10.1007/s11306-005-1108-2
Abstract
Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF) was applied for the analysis of complex metabolite profiles from mouse spleen. The resulting two-dimensional chromatograms proved that mass spectral quality and sensitivity were largely improved by the enhanced resolution and zone compression, which are features of GC × GC operation, when compared to classical one-dimensional GC-TOF methods. The improved peak capacity of GC × GC allowed for peaks to be detected that could previously not be separated in one-dimensional GC. A measure of the combined power of chromatographic and mass spectral deconvolution resolution is called "analytical purity", with higher values indicating less pure peaks. GC × GC-TOF lead to the detection of 1200 compounds with purity better than 0.2, compared to 500 compounds with purity up to 2.5 in one-dimensional GC-TOF. The compounds identified include many of the compounds previously reported in NMR studies and other methods on mammalian tissues. Spleen samples of several obese NZO mice and lean C57BL/6 control strains were analyzed in order to demonstrate the applicability of GC × GC-TOF for biomarker identification. © 2005 Springer Science+Business Media, Inc
Item Details
Item Type: | Refereed Article |
---|---|
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Manufacturing |
Objective Group: | Instrumentation |
Objective Field: | Scientific instruments |
UTAS Author: | Shellie, RA (Associate Professor Robert Shellie) |
ID Code: | 36241 |
Year Published: | 2005 |
Web of Science® Times Cited: | 129 |
Deposited By: | Chemistry |
Deposited On: | 2005-11-17 |
Last Modified: | 2012-04-20 |
Downloads: | 0 |
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