Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF) was applied for the analysis of complex metabolite profiles from mouse spleen. The resulting two-dimensional chromatograms proved that mass spectral quality and sensitivity were largely improved by the enhanced resolution and zone compression, which are features of GC × GC operation, when compared to classical one-dimensional GC-TOF methods. The improved peak capacity of GC × GC allowed for peaks to be detected that could previously not be separated in one-dimensional GC. A measure of the combined power of chromatographic and mass spectral deconvolution resolution is called "analytical purity", with higher values indicating less pure peaks. GC × GC-TOF lead to the detection of 1200 compounds with purity better than 0.2, compared to 500 compounds with purity up to 2.5 in one-dimensional GC-TOF. The compounds identified include many of the compounds previously reported in NMR studies and other methods on mammalian tissues. Spleen samples of several obese NZO mice and lean C57BL/6 control strains were analyzed in order to demonstrate the applicability of GC × GC-TOF for biomarker identification. © 2005 Springer Science+Business Media, Inc

Item Type:	Refereed Article
Research Division:	Chemical Sciences
Research Group:	Analytical Chemistry
Research Field:	Separation Science
Objective Division:	Manufacturing
Objective Group:	Instrumentation
Objective Field:	Scientific Instruments
Author:	Shellie, RA (Associate Professor Robert Shellie)
ID Code:	36241
Year Published:	2005
Web of Science® Times Cited:	104
Deposited By:	Chemistry
Deposited On:	2005-11-17
Last Modified:	2012-04-20
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