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Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC GC-TOF) for high resolution metabolomics: Biomarker discovery on spleen tissue extracts of obese NZO compared to lean C57BL/6 mice

Citation

Welthagen, W and Shellie, RA and Spranger, J and Ristow, M and Zimmerman, R and Fiehn, O, Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC GC-TOF) for high resolution metabolomics: Biomarker discovery on spleen tissue extracts of obese NZO compared to lean C57BL/6 mice, Metabolomics, 1, (1) pp. 65-73. ISSN 1573-3882 (2005) [Refereed Article]

DOI: doi:10.1007/s11306-005-1108-2

Abstract

Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC GC-TOF) was applied for the analysis of complex metabolite profiles from mouse spleen. The resulting two-dimensional chromatograms proved that mass spectral quality and sensitivity were largely improved by the enhanced resolution and zone compression, which are features of GC GC operation, when compared to classical one-dimensional GC-TOF methods. The improved peak capacity of GC GC allowed for peaks to be detected that could previously not be separated in one-dimensional GC. A measure of the combined power of chromatographic and mass spectral deconvolution resolution is called "analytical purity", with higher values indicating less pure peaks. GC GC-TOF lead to the detection of 1200 compounds with purity better than 0.2, compared to 500 compounds with purity up to 2.5 in one-dimensional GC-TOF. The compounds identified include many of the compounds previously reported in NMR studies and other methods on mammalian tissues. Spleen samples of several obese NZO mice and lean C57BL/6 control strains were analyzed in order to demonstrate the applicability of GC GC-TOF for biomarker identification. 2005 Springer Science+Business Media, Inc

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Manufacturing
Objective Group:Instrumentation
Objective Field:Scientific Instruments
Author:Shellie, RA (Associate Professor Robert Shellie)
ID Code:36241
Year Published:2005
Web of Science® Times Cited:108
Deposited By:Chemistry
Deposited On:2005-11-17
Last Modified:2012-04-20
Downloads:0

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