eCite Digital Repository

Statistical methods for comparing comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry results: Metabolomic analysis of mouse tissue extracts

Citation

Shellie, RA and Welthagen, W and Zrostlikova, J and Spranger, J and Ristow, M and Fiehn, O and Zimmermann, R, Statistical methods for comparing comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry results: Metabolomic analysis of mouse tissue extracts, Journal of Chromatography A, 1086, (1-2) pp. 83-90. ISSN 0021-9673 (2005) [Refereed Article]

DOI: doi:10.1016/j.chroma.2005.05.088

Abstract

The potential for the utilization of GC GC-time-of-flight (TOF) MS for high-resolution metabolomics studies is discussed, with the implementation of some statistical comparisons for biomarker detection. Metabolite profiles from NZO obese mice versus BL/6 control mice are compared and contrasted using a number of chromatogram comparison routines, including direct chromatogram comparisons, chromatogram subtraction and averaging routines, as well as a method for generating relative weighted peak surface difference chromatograms, and a more conventional Student's t-test statistical approach.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Manufacturing
Objective Group:Instrumentation
Objective Field:Scientific Instruments
Author:Shellie, RA (Associate Professor Robert Shellie)
ID Code:36240
Year Published:2005
Web of Science® Times Cited:106
Deposited By:Chemistry
Deposited On:2007-11-02
Last Modified:2012-04-20
Downloads:0

Repository Staff Only: item control page