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Proteomic Analysis of Myrmecia pilosula (Jack Jumper) Ant Venom

Citation

Wiese, MD and Chataway, TK and Davies, NW and Milne, RW and Brown, SGA and Gai, WP and Heddle, RJ, Proteomic Analysis of Myrmecia pilosula (Jack Jumper) Ant Venom, Toxicon, 47, (2) pp. 208-217. ISSN 0041-0101 (2006) [Refereed Article]


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DOI: doi:10.1016/j.toxicon.2005.10.018

Abstract

Ant sting allergy in Australia is predominantly due to the Myrmecia pilosula species complex. Gel separation of M. pilosula venom is necessary so that the allergenic importance of each component can be defined by western blotting. However, previous PAGE methods produced suboptimal resolution and the components of each band were not precisely defined. Venom was resolved in both non-reduced and reduced form by one-dimensional acid urea PAGE, SDS-PAGE and two-dimensional acid urea-SDS PAGE. Resolved peptides were extracted and analysed by HPLC–MS. Acid urea PAGE and acid urea-SDS PAGE proved more effective than SDS-PAGE for resolution of peptides smaller than 10 kDa. All of the major peptides previously observed in M. pilosula venom were observed in gel resolved venom. Venom was found to primarily consist of peptides with molecular weight <10 kDa, most of which contain disulfide bridges. SDS-PAGE of non-reduced venom clearly defined six higher molecular weight proteins between 26 and 90 kDa. An 8546 Da dimer named pilosulin 5 was observed, but pilosulin 4, a peptide recently proposed to be present in venom was not. A variant of pilosulin 4 here named pilosulin 4.1a, existing as an 8198 Da dimer, was observed and has been characterised.

Item Details

Item Type:Refereed Article
Keywords:Myrmecia pilosula; Jack jumper ant venom; SDS-PAGE; Acid urea PAGE; Mass spectrometry; Pilosulin 3; Pilosulin 4; Pilosulin 4.1; Pilosulin 5
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Allergy
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Immune System and Allergy
Author:Davies, NW (Associate Professor Noel Davies)
ID Code:36235
Year Published:2006
Web of Science® Times Cited:30
Deposited By:Central Science Laboratory
Deposited On:2006-08-01
Last Modified:2009-11-11
Downloads:0

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