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The identification of a barely haze active protein that influence beer haze stability: The genetic basis of a barley malt haze active protein

Citation

Robinson, LH and Healy, P and Stewart, DC and Eglinton, JK and Ford, CM and Evans, E, The identification of a barely haze active protein that influence beer haze stability: The genetic basis of a barley malt haze active protein, Journal of Cereal Science, 45, (3) pp. 335-342. ISSN 0733-5210 (2007) [Refereed Article]

DOI: doi:10.1016/j.jcs.2006.08.013

Abstract

In bright beers, the formation of haze is a serious quality problem, which places limitations on the storage life of the product. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) immunoblot analysis using an antiserum that was raised against a silica eluate (SE) protein fraction (obtained from silica gel, used for the colloidal stabilisation of beer), detected a range of protein bands in barley, malt, beer and haze. A polymorphism was observed in which some barley varieties contained a molecular weight (MW) ~12,000 band (SE +ve) while in other varieties this band was absent (SE -ve). A survey of 219 Australian and international barley varieties, including a comprehensive selection of current and past malting varieties, identified 181 varieties as SE +ve, and 38 varieties as SE -ve. Previous pilot brewing trials demonstrated that SE -ve varieties are desirable as the beer brewed from the malt of these varieties formed less haze after accelerated ageing than beers brewed using SE +ve malt varieties. The genetic basis for the absence of the SE protein was conferred by a recessive allele at a single locus. Interval mapping analysis showed that the MW ~12,000 band mapped to the short arm of chromosome 3H.

Item Details

Item Type:Refereed Article
Keywords:malt, protein, QTL
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Biochemistry and Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
Author:Evans, E (Dr Evan Evans)
ID Code:36222
Year Published:2007
Web of Science® Times Cited:26
Deposited By:Agricultural Science
Deposited On:2007-08-01
Last Modified:2013-01-31
Downloads:0

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