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The identification of a barley haze active protein that influences beer haze stability: Cloning and characterisation of the barley SE protein as a barley trypsin inhibitor of the chloroform/methanol type
Citation
Robinson, LH and Juttner, J and Milligan, A and Lahnstein, J and Eglinton, JK and Evans, E, The identification of a barley haze active protein that influences beer haze stability: Cloning and characterisation of the barley SE protein as a barley trypsin inhibitor of the chloroform/methanol type, Journal of Cereal Science, 45, (3) pp. 343-352. ISSN 0733-5210 (2007) [Refereed Article]
DOI: doi:10.1016/j.jcs.2006.08.012
Abstract
Previous pilot brewing trials have demonstrated that in the absence of a molecular weight (MW) ~12,000 (barley silica eluate (SE) protein), the beer brewed from the malt of these SE -ve varieties formed less haze after accelerated ageing than beers brewed using SE +ve malt varieties. The previously described SE protein was characterised using comparative two-dimensional (2-D) gel electrophoresis immunoblots of barley seed extracts from both SE +ve and SE -ve varieties. The SE protein spot identified was excised and its partial sequence determined, after in-gel cleavage using trypsin and separation of the resulting fragments by reversed-phase high performance liquid chromatography (HPLC). N-terminal sequence analysis of the tryptic peptides from SE +ve and SE -ve varieties identified the SE protein as the barley trypsin inhibitor-CMe precursor (BTI-CMe). The mature BTI-CMe protein is 13.3 kDa and its functional gene is located on chromosome 3H. Cloning of the BTI-CMe protein demonstrated that both SE -ve and SE +ve barley varieties contain a BTI-CMe protein family member that is similar but has a consistently different sequence, primarily in the last 30 amino acid residues of their C-termini.
Item Details
Item Type: | Refereed Article |
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Keywords: | barley trypsin inhibitor, malt, protein, beer, haze |
Research Division: | Agricultural, Veterinary and Food Sciences |
Research Group: | Crop and pasture production |
Research Field: | Crop and pasture biochemistry and physiology |
Objective Division: | Plant Production and Plant Primary Products |
Objective Group: | Grains and seeds |
Objective Field: | Barley |
UTAS Author: | Evans, E (Dr Evan Evans) |
ID Code: | 36221 |
Year Published: | 2007 |
Funding Support: | Australian Research Council (LP0560329) |
Web of Science® Times Cited: | 32 |
Deposited By: | Agricultural Science |
Deposited On: | 2007-08-01 |
Last Modified: | 2013-01-31 |
Downloads: | 0 |
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