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Stems of the Arabidopsis pin1-1 mutant are not deficient in free indole-3-acetic acid


Jones, SE and Demeo, JS and Davies, NW and Noonan, SE and Ross, JJ, Stems of the Arabidopsis pin1-1 mutant are not deficient in free indole-3-acetic acid, Planta, 222, (3) pp. 530-534. ISSN 0032-0935 (2005) [Refereed Article]

DOI: doi:10.1007/s00425-005-1561-1


The pin1-1 mutant of Arabidopsis thaliana has been pivotal for studies on auxin transport and on the role of auxin in plant development. It was reported previously that when whole shoots were analysed, levels of the major auxin, indole-3-acetic acid (IAA) were dramatically reduced in the mutant, compared with the WT (Okada et al. 1991). The cloning of PIN1, however, provided evidence that this gene encodes a facilitator of auxin efflux, raising the question of how the pin1-1 mutation might reduce overall IAA levels as well as IAA transport. We therefore re-examined IAA levels in individual parts of pin1-1 and WT plants, focusing on inflorescence stems. Our data show that there is in fact no systemic IAA deficiency in the mutant. The previously reported difference between mutant and WT may have been due to the inclusion of reproductive structures in the WT harvest: we show here that the inflorescence itself contains high levels of IAA. We reconcile the normal IAA levels of pin1-1 inflorescence stems with their (previously-reported) reduced ability to transport IAA by presenting evidence that the auxin in mutant stems is not imported from their apical portion. Our data also indicate that levels of another auxin, indole-3-butyric acid (IBA), are very low in stems of the genotypes used in this study. © Springer-Verlag 2005.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Jones, SE (Miss Sarah Jones)
UTAS Author:Demeo, JS (Mr Demeo)
UTAS Author:Davies, NW (Associate Professor Noel Davies)
UTAS Author:Noonan, SE (Ms Sophie Noonan)
UTAS Author:Ross, JJ (Associate Professor John Ross)
ID Code:36211
Year Published:2005
Web of Science® Times Cited:21
Deposited By:Plant Science
Deposited On:2005-08-01
Last Modified:2006-05-09

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