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The brassinosteroid growth response in pea is not mediated by changes in gibberellin content

Citation

Jager, C and Symons, GM and Ross, JJ and Smith, JJ and Reid, JB, The brassinosteroid growth response in pea is not mediated by changes in gibberellin content, Planta, 221, (1) pp. 141-148. ISSN 0032-0935 (2005) [Refereed Article]

DOI: doi:10.1007/s00425-004-1454-8

Abstract

The objective of this study was to increase our understanding of the relationship between brassinosteroids (BRs) and gibberellins (GAs) by examining the effects of BR deficiency on the GA biosynthesis pathway in several tissue types of pea (Pisum sativum L.). It was suggested recently that, in Arabidopsis, BRs act as positive regulators of GA 20-oxidation, a key step in GA biosynthesis [Bouquin et al. (2001) Plant Physiol 127:450-458]. However, this may not be the case in pea as GA20 levels were consistently higher in all shoot tissues of BR-deficient (lk and lkb) and BR-response (lka) mutants. The application of brassinolide (BL) to lkb plants reduced GA20 levels, and metabolism studies revealed a reduced conversion of GA19 to GA20 in epi-BL-treated lkb plants. These results indicate that BRs actually negatively regulate GA20 levels in pea. Although GA 20 levels are affected by BR levels, this does not result in consistent changes in the level of the bioactive GA, GA1. Therefore, even though a clear interaction exists between endogenous BR levels and the level of GA20, this interaction may not be biologically significant. In addition to the effect of BRs on GA levels, the effect of altered GA 1 levels on endogenous BR levels was examined. There was no significant difference in BR levels between the GA mutants and the wild type (wt), indicating that altered GA1 levels have no effect on BR levels in pea. It appears that the BR growth response is not mediated by changes in bioactive GA levels, thus providing further evidence that BRs are important regulators of stem elongation. © Springer-Verlag 2004.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Environmentally Sustainable Plant Production
Objective Field:Environmentally Sustainable Plant Production not elsewhere classified
Author:Jager, C (Dr Corinne Hamilton)
Author:Symons, GM (Mr Gregory Symons)
Author:Ross, JJ (Associate Professor John Ross)
Author:Smith, JJ (Ms Jenny Smith)
Author:Reid, JB (Professor Jim Reid)
ID Code:36118
Year Published:2005
Web of Science® Times Cited:35
Deposited By:Plant Science
Deposited On:2005-08-01
Last Modified:2006-05-08
Downloads:0

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