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Isolation of denitrifying bacteria from hydrocarbon-contaminated Antarctic soil

Citation

Powell, S and Ma, WK and Siciliano, SD, Isolation of denitrifying bacteria from hydrocarbon-contaminated Antarctic soil, Polar Biology, 30, (1) pp. 69-74. ISSN 0722-4060 (2006) [Refereed Article]

DOI: doi:10.1007/s00300-006-0161-2

Abstract

In this study, we report the isolation of denitrifiers from hydrocarbon-contaminated Antarctic soils. Seventy-two isolates were obtained from soils that had received a fertilizer treatment to stimulate hydrocarbon degradation. All isolates, except one, belonged to the genus Pseudomonas. The one exception was a member of the Microbacteriaceae, which was also, coincidentally, the only isolate negative for the nirS gene. The diversity of the 16S rRNA and nosZ genes was assessed by denaturing gradient gel electrophoresis and sequencing. There was a slight correlation between the 16S rRNA and nosZ operational taxonomic units. Surprisingly, many isolates contained nosZ on plasmids and, to the best of our knowledge, this is the first report of nosZ being extra-chromosomally present in Pseudomonas spp. © 2006 Springer-Verlag.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Powell, S (Dr Shane Powell)
ID Code:35973
Year Published:2006
Web of Science® Times Cited:5
Deposited By:Agricultural Science
Deposited On:2006-08-01
Last Modified:2007-04-17
Downloads:0

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