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Effects of melatonin on liver estrogen receptor and vitellogenin expression in rainbow trout: An in vitro and in vivo study

Citation

Mazurais, D and Porter, MJR and Lethimonier, C and Le Drean, G and Le Goff, P and Randall, C and Pakdel, F and Bromage, N and Kah, O, Effects of melatonin on liver estrogen receptor and vitellogenin expression in rainbow trout: An in vitro and in vivo study, General and comparative Endocrinology, 118, (2) pp. 344-353. ISSN 0016-6480 (2000) [Refereed Article]

DOI: doi:10.1006/gcen.2000.7472

Abstract

Although melatonin is believed to mediate many seasonal and circadian effects of photoperiod on reproduction in salmonids, the precise mechanisms underlying such effects are still largely unknown. Recent data of the literature indicate a relationship between melatonin and expression of estrogen receptors (ER) in various tissues. In this study, the effects of melatonin on estrogen receptor and/or vitellogenin expression were studied by a combination of in vivo and in vitro experiments. In yeast stably expressing ER and transfected with an estrogen-responsive element-β-galactosidase reporter gene, melatonin had no effect on basal or E2-stimulated ER expression. Incubation of hepatocyte aggregates with melatonin (10-8 to 10-4) for 16 or 48 h did not modify the E2-stimulated ER and vitellogenin mRNA, as measured by dot blots. Finally, neither pinealectomy nor melatonin implants caused any effect on basal or E2-stimulated ER and vitellogenin mRNA contents in the liver. Altogether, these results suggest that, although we cannot exclude potential effects at the brain or pituitary levels, melatonin has no or little effects on estrogen receptor in the liver. (C) 2000 Academic Press.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Porter, MJR (Dr Mark Porter)
ID Code:35150
Year Published:2000
Web of Science® Times Cited:13
Deposited By:TAFI - Aquaculture
Deposited On:2005-08-03
Last Modified:2011-10-06
Downloads:0

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